Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ptx3 Double Nickase Plasmid (h): sc-402766-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ptx3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ptx3 Double Nickase Plasmid (h) and Ptx3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PTX3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ptx3 Antibody (C-10): sc-373951
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ptx3 Double Nickase Plasmid (h) | sc-402766-NIC | 20 µg | $410.00 | |||
Ptx3 Double Nickase Plasmid (h2) | sc-402766-NIC-2 | 20 µg | $410.00 |
PTX3 encodes pentraxin 3 (Ptx3), a soluble pattern-recognition molecule of the long pentraxin family that participates in humoral innate immunity and extracellular matrix remodeling. Ptx3 is rapidly induced by inflammatory cues such as IL-1 and TNF and contributes to complement regulation by interacting with components including C1q and factor H, thereby shaping opsonization and inflammatory amplification. It also binds select microbial ligands and matrix proteins, linking pathogen sensing to tissue damage responses, angiogenic regulation, and leukocyte recruitment. Dysregulated PTX3 expression has been associated with inflammatory disorders, infection susceptibility, vascular pathology, and tumor-associated inflammation, making it a useful node for studying innate immune signaling and microenvironmental crosstalk.
Ptx3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PTX3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PTX3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PTX3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PTX3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.