PTPN21 Double Nickase Plasmid (h): sc-407466-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PTPN21 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PTPN21 Double Nickase Plasmid (h) and PTPN21 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PTPN21. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PTPN21 Double Nickase Plasmid (h) | sc-407466-NIC | 20 µg | $410.00 | |||
PTPN21 Double Nickase Plasmid (h2) | sc-407466-NIC-2 | 20 µg | $410.00 |
PTPN21 encodes a non-receptor protein tyrosine phosphatase that modulates signal transduction by dephosphorylating phosphotyrosine residues on target proteins, thereby tuning the amplitude and duration of receptor- and adhesion-linked pathways. It has been implicated in coordination of cytoskeletal remodeling, cell motility, and membrane trafficking through interactions with scaffolding and endocytic components, influencing processes such as focal adhesion dynamics and growth factor receptor turnover. Through these pathway connections, PTPN21 is frequently studied in contexts where tyrosine phosphorylation balance impacts proliferation, migration, and cellular stress responses. Altered regulation of phosphatase activity and tyrosine signaling networks involving PTPN21 has been associated with oncogenic signaling states and other diseases characterized by dysregulated cell signaling and adhesion.
PTPN21 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PTPN21 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PTPN21. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PTPN21 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PTPN21-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.