PTP-PEST Antibody (F-12): sc-398775
- PTP-PEST Antibody (F-12) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 747-775 near the C-terminus of PTP-TEST of mouse origin
- recommended for detection of PTP-TEST of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG3 BP-HRP is the preferred secondary detection reagent for PTP-PEST Antibody (F-12) for WB applications. This reagent is now offered in a bundle with PTP-PEST Antibody (F-12) (see ordering information below).
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PTP-PEST Antibody (F-12) is a mouse monoclonal IgG3 antibody that detects PTP-PEST in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PTP-PEST, also known as brain-derived phosphatase 1, plays a crucial role in regulating protein tyrosine phosphorylation, which is essential for various cellular processes including cell growth, migration, and differentiation. PTP-PEST is expressed in the brain, colon, and several tumor-derived cell lines, highlighting its significance in both normal physiology and cancer biology. PTP-PEST has been shown to selectively dephosphorylate autophosphorylated tyrosine kinases, such as Src and epidermal growth factor receptor (EGFR), which are often overexpressed in tumor tissues. PTP-PEST′s ability to modulate kinase activity underscores its potential as a therapeutic target and biomarker for cancer progression. Anti-PTP-PEST antibody (F-12) serves as an invaluable tool for researchers investigating cell signaling and tumor biology mechanisms.
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PTP-PEST Antibody (F-12) References:
- PTP-PEST, a scaffold protein tyrosine phosphatase, negatively regulates lymphocyte activation by targeting a unique set of substrates. | Davidson, D. and Veillette, A. 2001. EMBO J. 20: 3414-26. PMID: 11432829
- PTP-PEST couples membrane protrusion and tail retraction via VAV2 and p190RhoGAP. | Sastry, SK., et al. 2006. J Biol Chem. 281: 11627-36. PMID: 16513648
- PTP-PEST phosphatase variations in human cancer. | Streit, S., et al. 2006. Cancer Genet Cytogenet. 170: 48-53. PMID: 16965954
- Essential function of PTP-PEST during mouse embryonic vascularization, mesenchyme formation, neurogenesis and early liver development. | Sirois, J., et al. 2006. Mech Dev. 123: 869-80. PMID: 17070019
- Interaction of Pyk2 and PTP-PEST with leupaxin in prostate cancer cells. | Sahu, SN., et al. 2007. Am J Physiol Cell Physiol. 292: C2288-96. PMID: 17329398
- The phosphatase PTP-PEST promotes secondary T cell responses by dephosphorylating the protein tyrosine kinase Pyk2. | Davidson, D., et al. 2010. Immunity. 33: 167-80. PMID: 20727793
- Ras-induced and extracellular signal-regulated kinase 1 and 2 phosphorylation-dependent isomerization of protein tyrosine phosphatase (PTP)-PEST by PIN1 promotes FAK dephosphorylation by PTP-PEST. | Zheng, Y., et al. 2011. Mol Cell Biol. 31: 4258-69. PMID: 21876001
- Macrophage fusion is controlled by the cytoplasmic protein tyrosine phosphatase PTP-PEST/PTPN12. | Rhee, I., et al. 2013. Mol Cell Biol. 33: 2458-69. PMID: 23589331
- MVP-Associated Filamin A Mutations Affect FlnA-PTPN12 (PTP-PEST) Interactions. | Duval, D., et al. 2015. J Cardiovasc Dev Dis. 2: 233-247. PMID: 26594644
- Biochemical and Structural Studies of Protein Tyrosine Phosphatase PTP-PEST (PTPN12) in Search of Small Molecule Inhibitors. | Johnson, D., et al. 2025. Chem Biol Drug Des. 105: e70058. PMID: 39895370
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PTP-PEST Antibody (F-12) | sc-398775 | 200 µg/ml | $322.00 | |||
PTP-PEST Antibody (F-12): m-IgG3 BP-HRP Bundle | sc-550513 | 200 µg Ab; 40 µg BP | $361.00 | |||
PTP-PEST (F-12) Neutralizing Peptide | sc-398775 P | 100 µg/0.5 ml | $69.00 |