PTH-alanine (CAS 4333-19-1)
QUICK LINKS
PTH-alanine is a compound used in protein sequencing and analysis. It is a phenylthiohydantoin derivative of the amino acid alanine, which is employed in the Edman degradation sequencing method. During this process, PTH-alanine is produced when a peptide′s N-terminal alanine reacts with phenyl isothiocyanate under mildly acidic conditions and is subsequently cleaved from the peptide chain. Researchers utilize PTH-alanine to identify amino acid sequences in proteins and peptides by separating and quantifying PTH-amino acids using high-performance liquid chromatography (HPLC). The compound′s stability and distinct chromatographic properties facilitate the accurate determination of protein sequences. Additionally, PTH-alanine is valuable in proteomics for the characterization of complex protein mixtures, contributing to the understanding of protein structure and function.
PTH-alanine (CAS 4333-19-1) References
- Identification of PTH-amino acids by HPLC. | Grant, GA. and Crankshaw, MW. 2003. Methods Mol Biol. 211: 247-68. PMID: 12489436
- Structural characterization of lacticin 3147, a two-peptide lantibiotic with synergistic activity. | Martin, NI., et al. 2004. Biochemistry. 43: 3049-56. PMID: 15023056
- Histone H3 lysine 4 mono-methylation does not require ubiquitination of histone H2B. | Dehé, PM., et al. 2005. J Mol Biol. 353: 477-84. PMID: 16185711
- Electrochemical analysis of the alanine phenylthiohydantoin derivative by cathodic stripping voltammetry. | Vilaseca, C., et al. 2008. Anal Biochem. 379: 91-5. PMID: 18501695
- [24] Degradation of peptides by the Edman method with direct identification of the phenylthiohydantoin-amino acid. | Schroeder, WA. 1972. Methods Enzymol. 25: 298-313. PMID: 23014411
- Quantitative In Silico Analysis of Retention of Phenylthiohydantoin-Amino Acids in Reversed-Phase Ion-Pair Liquid Chromatography. | Hanai, T. 2016. J Chromatogr Sci. 54: 604-8. PMID: 26769717
- Thermodynamic enantioseparation behavior of phenylthiohydantoin-amino acid derivatives in supercritical fluid chromatography on polysaccharide chiral stationary phases. | Khater, S., et al. 2018. J Sep Sci. 41: 1450-1459. PMID: 29266870
- Reversed-phase high-performance liquid chromatographic separation and quantitation of phenylthiohydantoin derivatives of 25 amino acids, including those of cysteic acid, 4-hydroxyproline, methionine sulfone, S-carboxymethylcysteine and S-methylcysteine. | Kolbe, HV., et al. 1985. J Chromatogr. 327: 1-7. PMID: 4030953
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PTH-alanine, 100 mg | sc-236501 | 100 mg | $141.00 |