PtdIns-(5)-P1 (1,2-dioctanoyl) (ammonium salt)

PtdIns-(5)-P1 (1,2-dioctanoyl) ammonium salt is a synthetic analog of phosphatidylinositol-5-phosphate (PI5P) with short-chain dioctanoyl fatty acids at the sn-1 and sn-2 positions. This compound provides researchers a soluble analog for studying the regulatory mechanisms involving PI5P, a relatively minor but significant signaling lipid implicated in various cellular processes. The dioctanoyl chains increase solubility, making it suitable for use in micelles, liposomes, and biochemical assays. PI5P is a precursor for phosphoinositides that participate in cell signaling, particularly in stress responses, cytoskeletal organization, and nuclear signaling. PI5P interacts with proteins like ING2 (Inhibitor of Growth 2) and modulates the activity of several kinases and phosphatases. PtdIns-(5)-P1 (1,2-dioctanoyl) ammonium salt has been used in research to probe the enzymatic activity of kinases, such as PI5P4-kinases, which convert PI5P into PI(4,5)P2, and phosphatases like myotubularin. By serving as a substrate or competitor in enzyme assays, this compound helps explain the specificity and kinetics of PI5P metabolism. Furthermore, researchers have used it to investigate the localization, binding specificity, and regulatory effects of proteins interacting with PI5P, thereby clarifying their roles in intracellular signaling pathways.