PtdIns-(4)-P1 (1,2-dihexanoyl) sodium salt

PtdIns-(4)-P1 (1,2-dihexanoyl) sodium salt is a synthetic analog of phosphatidylinositol-4-phosphate, featuring short-chain hexanoyl fatty acids at the sn-1 and sn-2 positions. This compound serves as a key substrate in research for analyzing lipid signaling pathways. The dihexanoyl fatty acid chains confer increased solubility, simplifying incorporation into micelles and liposomes, enabling researchers to study enzyme kinetics, protein-lipid interactions, and membrane dynamics. A significant application is in investigating the role of phosphatidylinositol-4-phosphate (PI4P) in phosphoinositide-mediated signaling pathways. PI4P serves as a precursor for phosphatidylinositol-4,5-bisphosphate (PIP2), a critical signaling lipid in various cellular processes, such as cytoskeletal regulation, vesicle trafficking, and membrane organization. Researchers utilize PtdIns-(4)-P1 (1,2-dihexanoyl) sodium salt as a substrate to study the activity of lipid kinases like phosphatidylinositol-4-phosphate 5-kinases (PIP5Ks), which convert PI4P to PIP2. Additionally, it provides a model for exploring the enzymatic activity of phosphatases that regulate PI4P levels, such as Sac1 and other inositol polyphosphate phosphatases. Its defined chemical properties make it valuable for assessing enzyme kinetics, phosphoinositide-protein interactions, and the regulation of phosphoinositide signaling, allowing a deeper understanding of cellular signaling mechanisms.