PtdIns-(4,5)-P2 (1,2-dihexanoyl) (sodium salt)

PtdIns-(4,5)-P2 (1,2-dihexanoyl) sodium salt is a soluble analog of phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), a key phosphoinositide involved in numerous cellular signaling pathways. Its 1,2-dihexanoyl chains confer increased solubility, enabling its use in studying the dynamics of PI(4,5)P2-mediated signaling events. PI(4,5)P2 is synthesized by kinases that phosphorylate PI(4)P at the D5 position of the inositol ring, and it is involved in diverse cellular processes like actin cytoskeletal regulation, membrane trafficking, and signal transduction. This synthetic analog has been employed in research to probe the role of PI(4,5)P2 in recruiting and activating proteins with phosphoinositide-binding domains, particularly pleckstrin homology (PH) and FYVE domains. It is commonly used in competitive binding assays to explore how proteins like PLC-γ, dynamin, and N-WASP interact with PI(4,5)P2 in vivo. Additionally, it provides a reliable substrate for enzymatic studies investigating phosphoinositide kinases and phosphatases. In membrane trafficking studies, this compound aids in deciphering how PI(4,5)P2 regulates vesicle formation and endocytosis. It is also valuable in understanding the molecular basis of actin polymerization and how phosphoinositides spatially and temporally coordinate cytoskeletal reorganization. Researchers often utilize PtdIns-(4,5)-P2 (1,2-dihexanoyl) in liposome-based assays to recreate membrane environments and assess protein-lipid interactions.