PtdIns-(3,4)-P2 (1,2-dihexanoyl) (sodium salt)

PtdIns-(3,4)-P2 (1,2-dihexanoyl) sodium salt is a short-chain analog of phosphatidylinositol-3,4-bisphosphate (PI(3,4)P2), a significant phosphoinositide involved in cellular signaling pathways. The 1,2-dihexanoyl chains enhance its solubility, enabling researchers to study the role of PI(3,4)P2 in various signaling processes. PI(3,4)P2 is synthesized primarily through phosphorylation of PI(3)P by kinases and plays a critical role in recruiting effector proteins containing pleckstrin homology (PH) domains to cellular membranes. This synthetic analog provides a versatile tool for exploring PI(3,4)P2 signaling in vitro and in vivo. It can act as a substrate in enzymatic assays for characterizing kinase and phosphatase activity. For instance, its usage in competitive binding assays aids in deciphering the specific interactions of PH domain-containing proteins, such as TAPP1, TAPP2, and PKB/Akt, with PI(3,4)P2. Additionally, it is instrumental in studying the localization and dynamics of these proteins within cellular membranes. PtdIns-(3,4)-P2 (1,2-dihexanoyl) sodium salt also serves in biochemical assays to investigate the effects of PI(3,4)P2 on cytoskeletal reorganization, vesicular trafficking, and receptor signaling. Its use in reconstituted systems, liposomes, and cell lines helps illuminate the molecular mechanisms underlying PI(3,4)P2-mediated cellular processes.