PtdIns-(3,4,5)-P3-biotinamide (sodium salt) (CAS 1415684-80-8)

PtdIns-(3,4,5)-P3-biotinamide (sodium salt) is a modified form of phosphatidylinositol triphosphate (PIP3) that incorporates a biotinamide moiety. This chemical plays a crucial role in biochemical research, particularly in elucidating the signaling pathways mediated by PIP3. PIP3 is a key phosphoinositide lipid second messenger involved in diverse cellular processes, including cell proliferation, survival, and motility. By conjugating biotinamide to PIP3, researchers can selectively label and isolate PIP3-interacting proteins through streptavidin-based affinity purification methods. This enables the identification and characterization of downstream effectors and regulatory proteins involved in PIP3 signaling cascades. Additionally, PtdIns-(3,4,5)-P3-biotinamide facilitates the visualization and tracking of PIP3 localization within cellular membranes using fluorescence microscopy techniques. Its application extends to studying signal transduction pathways implicated in various physiological and pathological conditions, such as cancer, immune responses, and metabolic disorders. Furthermore, this chemical serves as a valuable tool in proteomics and lipidomics research, offering insights into the complex network of molecular interactions governing cellular signaling events mediated by PIP3. Overall, PtdIns-(3,4,5)-P3-biotinamide plays a pivotal role in advancing our understanding of phosphoinositide signaling and its implications in cellular physiology and disease.