PtdIns-(3,4,5)-P3 (1,2-dioctanoyl) (sodium salt)

PtdIns-(3,4,5)-P3 (1,2-dioctanoyl) sodium salt, a synthetic derivative of phosphatidylinositol trisphosphate (PIP3), serves as a valuable tool in elucidating the signaling pathways governed by phosphoinositides. PIP3, generated by the phosphorylation of PIP2 at the 3-position by phosphoinositide 3-kinase (PI3K), is a critical secondary messenger involved in various cellular processes, including cell growth, survival, and migration. This specific analog, with shorter acyl chains, enhances solubility and accessibility in experimental settings. In research, it has been extensively employed to dissect the downstream signaling cascades initiated by PIP3. Notably, PIP3 recruits signaling proteins containing pleckstrin homology (PH) domains, such as Akt and PDK1, to the plasma membrane, facilitating their activation and subsequent modulation of downstream effectors involved in cell proliferation and survival. Additionally, this analog has been instrumental in studying the spatiotemporal dynamics of PIP3 signaling within the cell, including its role in coordinating cytoskeletal rearrangements during cell migration and its involvement in regulating membrane trafficking events. Furthermore, researchers utilize this compound in competitive binding assays and fluorescence-based imaging studies to investigate the specific interactions and localization patterns of PIP3-binding proteins, providing crucial insights into the molecular mechanisms underlying cellular signaling pathways.