PSGL-1 Antibody (215): sc-32302
- PSGL-1 Antibody (215) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 200 µg/ml
- raised against P-selectin glycoprotein ligand-1 of human origin
- recommended for detection of PSGL-1 of human origin by FCM
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- See PSGL-1 (KPL1): sc-13535 for PSGL-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PSGL-1 Antibody (215). These reagents are now offered in bundles with PSGL-1 Antibody (215) (see ordering information below).
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SEE ALSO...
PSGL-1 Antibody (215) is a mouse monoclonal IgG2a antibody that detects PSGL-1 in human samples through flow cytometry (FCM). PSGL-1, also known as P-Selectin glycoprotein ligand or CD162, plays a crucial role in the immune response by mediating leukocyte adhesion to the endothelium during inflammation. PSGL-1 is primarily located on leukocyte microvilli tips, where a unique structure, characterized by a disulfide-linked homodimer and a rich extracellular domain composed of serines, threonines, and prolines, facilitates selectin interactions. The presence of 15 and 16 decameric repeats in HL-60 and U-937 cells, as well as in human leukocytes, enhances binding affinity to P-Selectin and E-Selectin, which is essential for leukocyte trafficking and recruitment to inflammation sites. For optimal selectin binding, PSGL-1 must undergo specific post-translational modifications, including sialylation and fucosylation, which are critical for function as a cell adhesion receptor. PSGL-1 belongs to a broader family of sialomucins, which transduce negative signals in hematopoietic cells, thereby influencing immune responses. Anti-PSGL-1 antibody (215) serves as an invaluable tool for researchers studying leukocyte behavior and inflammation mechanisms.
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PSGL-1 Antibody (215) References:
- PSGL-1 function in immunity and steady state homeostasis. | Carlow, DA., et al. 2009. Immunol Rev. 230: 75-96. PMID: 19594630
- PSGL-1-dependent myeloid leukocyte activation. | Zarbock, A., et al. 2009. J Leukoc Biol. 86: 1119-24. PMID: 19703898
- PSGL-1 Is an Immune Checkpoint Regulator that Promotes T Cell Exhaustion. | Tinoco, R., et al. 2016. Immunity. 44: 1190-203. PMID: 27192578
- The Interaction of Selectins and PSGL-1 as a Key Component in Thrombus Formation and Cancer Progression. | Kappelmayer, J. and Nagy, B. 2017. Biomed Res Int. 2017: 6138145. PMID: 28680883
- PSGL-1 restricts HIV-1 infectivity by blocking virus particle attachment to target cells. | Fu, Y., et al. 2020. Proc Natl Acad Sci U S A. 117: 9537-9545. PMID: 32273392
- Targeting the PSGL-1 Immune Checkpoint Promotes Immunity to PD-1-Resistant Melanoma. | DeRogatis, JM., et al. 2022. Cancer Immunol Res. 10: 612-625. PMID: 35303066
- PSGL-1 is a novel tumor microenvironment prognostic biomarker with cervical high-grade squamous lesions and more. | Lin, Y., et al. 2023. Front Oncol. 13: 1052201. PMID: 36969026
- Chemokine binding to PSGL-1 is controlled by O-glycosylation and tyrosine sulfation. | Goth, CK., et al. 2023. Cell Chem Biol. 30: 893-905.e7. PMID: 37463583
- PSGL-1 is an evolutionarily conserved antiviral restriction factor. | Jiang, C., et al. 2023. mBio. 14: e0038723. PMID: 37787515
- The role of P-selectin/PSGL-1 in regulating NETs as a novel mechanism in cerebral ischemic injury. | Li, X., et al. 2024. Front Neurol. 15: 1442613. PMID: 39022737
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSGL-1 Antibody (215) | sc-32302 | 200 µg/ml | $322.00 | |||
PSGL-1 Antibody (215): m-IgG Fc BP-HRP Bundle | sc-536735 | 200 µg Ab; 10 µg BP | $361.00 | |||
PSGL-1 Antibody (215): m-IgGκ BP-HRP Bundle | sc-533896 | 200 µg Ab; 40 µg BP | $361.00 | |||
PSGL-1 Antibody (215): m-IgG2a BP-HRP Bundle | sc-547862 | 200 µg Ab; 10 µg BP | $361.00 | |||
PSGL-1 Antibody (215) FITC | sc-32302 FITC | 200 µg/ml | $336.00 | |||
PSGL-1 Antibody (215) PE | sc-32302 PE | 200 µg/ml | $349.00 |