Pseudouridine-5′-triphosphate Sodium Salt (CAS 1175-34-4)

Pseudouridine-5′-triphosphate Sodium Salt is a chemical compound derived from pseudouridine, a naturally occurring modified nucleoside found in RNA. Scientifically, Pseudouridine-5′-triphosphate Sodium Salt finds applications in various fields of study. One notable area is RNA modification and epigenetics research. As a modified nucleoside triphosphate, it can be incorporated into RNA molecules during transcription or used as a substrate for RNA-modifying enzymes. By introducing pseudouridine into RNA, researchers can investigate its effects on RNA structure, stability, and function, leading to a deeper understanding of RNA biology. Furthermore, Pseudouridine-5′-triphosphate Sodium Salt has been utilized in the synthesis of labeled RNA for various applications, including RNA labeling for tracking and imaging studies. By incorporating this modified nucleotide into RNA sequences, scientists can track RNA molecules in cellular and molecular processes. The mechanism of action of Pseudouridine-5′-triphosphate Sodium Salt lies in its incorporation into RNA molecules. During transcription or in vitro RNA synthesis, it can be enzymatically added to the growing RNA chain by specific RNA-modifying enzymes. Once incorporated, pseudouridine can influence the structural conformation of RNA, affecting its stability, folding, and interactions with other molecules. This modification can impact RNA-protein interactions, RNA localization, and translation efficiency, thus altering the functional properties of the RNA molecule.