PRX II Double Nickase Plasmid (h): sc-401330-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRX II Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PRX II Double Nickase Plasmid (h) and PRX II Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PRDX2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRX II Antibody (A-2): sc-515428
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRX II Double Nickase Plasmid (h) | sc-401330-NIC | 20 µg | $410.00 | |||
PRX II Double Nickase Plasmid (h2) | sc-401330-NIC-2 | 20 µg | $410.00 |
PRDX2 encodes peroxiredoxin-2 (PRX II), a thiol-dependent peroxidase that reduces hydrogen peroxide and organic hydroperoxides using the thioredoxin system to maintain redox homeostasis. By shaping local H2O2 gradients, PRX II modulates redox-sensitive signaling that influences MAPK and NF-κB pathway dynamics, protein S-glutathionylation, and oxidative stress responses. In human cells, PRX II supports erythrocyte integrity and broader cytoprotective programs by limiting ROS-driven macromolecular damage and regulating apoptosis and inflammatory signaling. Altered PRDX2 expression or activity has been associated with contexts of oxidative stress and dysregulated redox signaling observed across cancer biology, neuroinflammation, and cardiometabolic disease models, supporting its use as a mechanistic node in redox pathway research.
PRX II Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PRDX2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PRDX2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PRDX2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PRDX2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.