Prothrombin Antibody (AHP-5013): sc-73470

Prothrombin Antibody (AHP-5013) is a mouse monoclonal IgG2a antibody that detects Prothrombin protein of human origin by western blotting (WB) and immunoprecipitation (IP). Anti-Prothrombin antibody (AHP-5013) is available as a non-conjugated format. Prothrombin, also known as coagulation factor II, plays a crucial role in the hemostatic process, serving as a precursor to Thrombin, which is essential for blood coagulation. Prothrombin is synthesized in the liver and primarily located in the plasma, where Prothrombin circulates in an inactive form until activated by proteolytic cleavage. This activation is vital, as Thrombin not only catalyzes the conversion of fibrinogen to fibrin, leading to clot formation, but also activates several other coagulation factors, thereby amplifying the coagulation cascade. The precise regulation of Prothrombin and its conversion to Thrombin is critical for maintaining hemostatic balance; dysregulation can lead to conditions such as thrombosis or bleeding disorders. Prothrombin monoclonal antibody (AHP-5013) is an invaluable tool for researchers studying coagulation pathways and related disorders, providing insights into the mechanisms of hemostasis and potential therapeutic targets.

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Prothrombin Antibody (AHP-5013) References:

1. Thrombin inhibits tumor cell growth in association with up-regulation of p21(waf/cip1) and caspases via a p53-independent, STAT-1-dependent pathway.  |  Huang, YQ., et al. 2000. J Biol Chem. 275: 6462-8. PMID: 10692450
2. The coagulation cascade: initiation, maintenance, and regulation.  |  Davie, EW., et al. 1991. Biochemistry. 30: 10363-70. PMID: 1931959
3. Basic mechanisms in blood coagulation.  |  Davie, EW. and Fujikawa, K. 1975. Annu Rev Biochem. 44: 799-829. PMID: 237463
4. Histone H4 promotes prothrombin autoactivation.  |  Barranco-Medina, S., et al. 2013. J Biol Chem. 288: 35749-57. PMID: 24178300
5. Proteolytic derivatives of thrombin.  |  Elion, J., et al. 1986. Ann N Y Acad Sci. 485: 16-26. PMID: 2436522
6. Prothrombin Complex Concentrates Only After Thromboelastography.  |  Muggleton, E. and Muggleton, T. 2019. Hepatol Commun. 3: 1013. PMID: 31304454
7. Prothrombin structure, activation, and biosynthesis.  |  Suttie, JW. and Jackson, CM. 1977. Physiol Rev. 57: 1-70. PMID: 319462
8. Human genes encoding prothrombin and ceruloplasmin map to 11p11-q12 and 3q21-24, respectively.  |  Royle, NJ., et al. 1987. Somat Cell Mol Genet. 13: 285-92. PMID: 3474786
9. Actions of thrombin and other coagulant and proteolytic enzymes on blood platelets.  |  Davey, MG. and Lüscher, EF. 1967. Nature. 216: 857-8. PMID: 6074962
10. Prothrombin complex concentrate.  |  DeWitt, RT. and Feinstein, DI. 1977. Arch Intern Med. 137: 1211-3. PMID: 901090
11. Thrombin stimulates fibroblast procollagen production via proteolytic activation of protease-activated receptor 1.  |  Chambers, RC., et al. 1998. Biochem J. 333 (Pt 1): 121-7. PMID: 9639571
12. Congenital deficiencies and abnormalities of prothrombin.  |  Girolami, A., et al. 1998. Blood Coagul Fibrinolysis. 9: 557-69. PMID: 9863703

The preferred Thrombin antibody is Thrombin Antibody (F-1): sc-271449.