Prothrombin Antibody (28H5): sc-69769
- Prothrombin Antibody (28H5) is a mouse monoclonal IgG2b provided at 100 µg/ml
- raised against Prothrombin of human origin
- recommended for detection of Prothrombin of human origin by WB, IP and ELISA
- See Thrombin (F-1): sc-271449 for Prothrombin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Prothrombin Antibody (28H5). This work is in progress.
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Prothrombin Antibody (28H5) is a mouse monoclonal IgG2b antibody that detects Prothrombin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Prothrombin, also known as Factor II, plays a critical role in the hemostatic process, serving as a precursor to Thrombin, which is essential for blood coagulation. The conversion of Prothrombin to Thrombin is initiated by proteolytic cleavage at residue 44, a crucial step that activates several other coagulation factors, including Factors V, VII, VIII, and XIII. This activation cascade not only facilitates the formation of fibrin clots but also enhances platelet aggregation, thereby preventing excessive bleeding following tissue injury. Additionally, Prothrombin is involved in maintaining vascular integrity during development and postnatal life, as Prothrombin coordinates the activity of connective tissue proteins by stimulating fibroblast production of procollagen. Prothrombin (28H5) antibody is an invaluable tool for researchers studying coagulation pathways and related disorders, providing insights into the mechanisms of hemostasis and potential therapeutic targets.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Prothrombin Antibody (28H5) References:
- Thrombogenicity of prothrombin complex concentrates. | Köhler, M. 1999. Thromb Res. 95: S13-7. PMID: 10499904
- Congenital prothrombin deficiency. | Lancellotti, S. and De Cristofaro, R. 2009. Semin Thromb Hemost. 35: 367-81. PMID: 19598065
- Hereditary prothrombin deficiency. | Ejaz, MS., et al. 2009. J Pak Med Assoc. 59: 637-9. PMID: 19750864
- Prothrombin fragments in cardiovascular disease. | Páramo, JA. 2010. Adv Clin Chem. 51: 1-23. PMID: 20857616
- Prothrombin complex concentrate. | Cada, DJ., et al. 2013. Hosp Pharm. 48: 951-7. PMID: 24474837
- Probing prothrombin structure by limited proteolysis. | Acquasaliente, L., et al. 2019. Sci Rep. 9: 6125. PMID: 30992526
- Using Plasma and Prothrombin Complex Concentrates. | Levy, JH., et al. 2020. Semin Thromb Hemost. 46: 32-37. PMID: 31537028
- Vitamin K and prothrombin synthesis. | Suttie, JW. 1973. Nutr Rev. 31: 105-9. PMID: 4578889
- The biology of prothrombin. | Degen, SJ. and Sun, WY. 1998. Crit Rev Eukaryot Gene Expr. 8: 203-24. PMID: 9714898
- Prothrombin as cofactor for antiphospholipids. | Galli, M. and Barbui, T. 1998. Lupus. 7 Suppl 2: S37-40. PMID: 9814670
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Prothrombin Antibody (28H5) | sc-69769 | 100 µg/ml | $322.00 |