Date published: 2025-9-25
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Protein A/G PLUS-Agarose: sc-2003
Protein A/G PLUS-Agarose: sc-2003
Protein A/G PLUS-Agarose: sc-2003
Protein A/G PLUS-Agarose: sc-2003
Laminin γ-1 (B-4) Alexa Fluor® 488: sc-13144 AF488. Direct fluorescent western blot analysis of Laminin γ-1 expression in
Laminin γ-1 (B-4) Alexa Fluor® 488: sc-13144 AF488. Direct fluorescent western blot analysis of Laminin γ-1 expression in HeLa whole cell lysate (A) and HeLa whole cell lysate immunoprecipitated with Laminin γ-1 (B-4): sc-13144 (B). Blocked with UltraCruz® Blocking Reagent: sc-516214. Immunoprecipitation reagent used: Protein A/G-PLUS-Agarose: sc-2003.
Protein A/G PLUS-Agarose: sc-2003

Protein A/G PLUS-Agarose: sc-2003

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Datasheets
  • Provides binding for all species of antibody and IgG subclasses recognized by either Protein A or Protein G
  • provided as 0.5 ml agarose/2.0 ml; 100 reactions
  • suitable for use at 20 µl per immunoprecipitation reaction
  • binding capacity is 10 mg of antibody per 1 ml of packed beads, settled not spun
  • pre-treated to reduce non-specific immunoglobulin binding
  • linked to Sepharose CL-6B
  • strong binding to mouse IgG1, mouse IgG2a, IgG2b and IgG3, rat IgG2a and IgG2c, human IgG1, IgG2, IgG3 and IgG4, rabbit, goat, bovine, porcine, ovine, canine, feline and equine IgG
  • weak to medium binding to rat IgG1, rat IgG2b, human IgM, IgA and IgE
  • pre-washed and ready to use

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Description
Datasheets and Protocols

SEE ALSO...

Molecular purification methods such as immunoprecipitation (IP), co-IP and chromatin IP are important tools in studying lowly expressed proteins, protein-protein interactions or protein-nucleic acid interactions. Santa Cruz Biotechnology offers a comprehensive array of research support products for purification techniques. Protein A/G PLUS is an essential specialty reagent used for isolating immunoglobulins from nearly any mammalian species. Protein A/G PLUS is a genetically-engineered protein that combines binding profiles of Protein A and Protein G, thereby producing an exceptionally high-capacity reagent. Protein A/G PLUS works through non-covalent high affinity binding of either protein A or protein G to the Fc regions of mammalian antibody isotypes, specifically IgG, IgA, IgE and IgM. Protein A/G PLUS is a powerful reagent for purification and detection of mouse monoclonal antibodies from IgG subclasses without interference from other serum proteins. Protein A/G PLUS is linked to a high-quality Agarose resin, CL-6B, which makes it a superior and premium product that produces high purity and purification yield of IgGs from mammalian samples.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Protein A/G PLUS-Agarose References:

  1. Tyrosine phosphorylation of Shc proteins in normal CD34+ progenitor cells and leukemic cells.  |  Yang, J., et al. 1999. Blood. 94: 373-4. PMID: 10428548
  2. Silibinin causes cell cycle arrest and apoptosis in human bladder transitional cell carcinoma cells by regulating CDKI-CDK-cyclin cascade, and caspase 3 and PARP cleavages.  |  Tyagi, A., et al. 2004. Carcinogenesis. 25: 1711-20. PMID: 15117815
  3. Mechanisms involved in resveratrol-induced apoptosis and cell cycle arrest in prostate cancer-derived cell lines.  |  Benitez, DA., et al. 2007. J Androl. 28: 282-93. PMID: 17050787
  4. DNA damage induced by multiwalled carbon nanotubes in mouse embryonic stem cells.  |  Zhu, L., et al. 2007. Nano Lett. 7: 3592-7. PMID: 18044946
  5. Antigen-subtracted 2-DE/MS strategy, a novel proteomic analysis platform.  |  Zhao, P., et al. 2011. Arch Toxicol. 85: 35-41. PMID: 20407759
  6. Determination of histone acetylation status by chromatin immunoprecipitation.  |  Galdieri, L., et al. 2012. Methods Mol Biol. 809: 255-65. PMID: 22113281
  7. Generation and characterization of polyclonal antibodies against mouse T-cell immunoglobulin and immunoreceptor tyrosine-based inhibitory domain by DNA-based immunization.  |  Gao, Y., et al. 2014. Transplant Proc. 46: 260-5. PMID: 24507063
  8. Proteomic analysis of Fasciola hepatica excretory and secretory products (FhESPs) involved in interacting with host PBMCs and cytokines by shotgun LC-MS/MS.  |  Liu, Q., et al. 2017. Parasitol Res. 116: 627-635. PMID: 27866265
  9. Characterization of a Protein Interactome by Co-Immunoprecipitation and Shotgun Mass Spectrometry.  |  Maccarrone, G., et al. 2017. Methods Mol Biol. 1546: 223-234. PMID: 27896772
  10. The EMSY threonine 207 phospho-site is required for EMSYdriven suppression of DNA damage repair.  |  Jelinic, P., et al. 2017. Oncotarget. 8: 13792-13804. PMID: 28099152
  11. Biomarker analysis of fucosylated kininogen through depletion of lectin reactive heterophilic antibodies in hepatocellular carcinoma.  |  Wang, M., et al. 2018. J Immunol Methods. 462: 59-64. PMID: 30144410
  12. Analysis of PD-L1 Transcriptional Regulation in Ovarian Cancer Cells by Chromatin Immunoprecipitation.  |  Zou, Y., et al. 2020. Methods Mol Biol. 2108: 229-239. PMID: 31939185
  13. Genistein suppresses ox-LDL-elicited oxidative stress and senescence in HUVECs through the SIRT1-p66shc-Foxo3a pathways.  |  Zhang, H., et al. 2022. J Biochem Mol Toxicol. 36: e22939. PMID: 34719845
  14. Inhibitors of p38 mitogen-activated protein kinase promote neuronal survival in vitro.  |  Horstmann, S., et al. 1998. J Neurosci Res. 52: 483-90. PMID: 9589393

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Protein A/G PLUS-Agarose

sc-2003
2 ml
$63.00

琼脂糖磁珠如何洗涤

Asked by: YAQIAN
Thanks for your question. sc-2003 is pre-washed and ready to use. Here is the link to our IP protocol:https://www.scbt.com/zh/resources/protocols/immunoprecipitation-western-blots. 感谢您的提问。sc-2003是预洗，即用型。以下是我们建议额IP实验流程供参考：https://www.scbt.com/zh/resources/protocols/immunoprecipitation-western-blots。
Answered by: Yanru Tao
Date published: 2025-08-08

Hi! Are those beads suitable for mass spec analysis of immunoprecipitated protein?

Asked by: layoa
Thank you for your question. Yes, these beads are suitable for immunoprecipitation with downstream mass spectrometry analysis.
Answered by: Tech Support Europe
Date published: 2025-07-02

The diameter of Protein A/G PLUS-Agarose(sc-2003), please?

Asked by: daodao
Thanks for your question. Protein G: 91 micro-meters. Protein A: 45-155 micro-meters. 感谢您的问题。蛋白质G: 91微米。蛋白质A: 45-155微米
Answered by: Yanru Tao
Date published: 2025-08-07

We are looking to purchase an antibody produced by Santa Cruz and used in this paper in the year 2005 - Journal of Cellular Biochemistry 95:1029–1041 (2005) Receptor Activator of NF-kB Ligand Protein Expression in UMR-106 Cells Is Differentially Regulated

Asked by: Whitney P
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service. Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-02-07

Does the stock solution contain Ethanol and as such, do the beads need to be washed before use? If not, which preservatives does the stock solution contain?

Asked by: khra
Thank you for your question. You can find our recommended immunoprecipitation protocol under this link: https://www.scbt.com/resources/protocols/immunoprecipitation-western-blots
Answered by: Tech Support Europe
Date published: 2025-01-17

Which non-denaturing elution buffer would you recommend with these beads? And which bead suspension:elution buffer ratio would you recommend?

Asked by: khra
Thank you for your question. You can find our recommended immunoprecipitation protocol under this link: https://www.scbt.com/resources/protocols/immunoprecipitation-western-blots
Answered by: Tech Support Europe
Date published: 2025-01-17

did you check endotoxins for this product/batch? we are looking for a Protein A free of endotoxins. many thanks

Asked by: evaa
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service. please email scbt@scbt.com or call 800-457-3801.
Answered by: BlakeJ
Date published: 2025-04-21

I will use them(sc-2003) for CHIP assay. I'd like to know whether the Protein A/G PLUS-Agarose are pre-blocked? Do I need to block them before using and could you please make it clear what has been used to pre-block them？

Asked by: lll890
Thank you for your question. Protein A/G PLUS-Agarose is pre-blocked with BSA to reduce non-specific immunoglobulin binding. If you have any other question, please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Sen Li
Date published: 2024-09-02
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Rated 5 out of 5 by from Perfect for co-immunoprecipitation.I had performed co-immunoprecipitation for the first time by using the Agarose A/G beads. my pulldown yield was very good. Along with that the protocol very easy and user friendly such that even beginners can do it.
Date published: 2024-12-05
Rated 5 out of 5 by from Research ScientistIt arrived ( to Canada) next day and worked well for immunoprecipitation (IP). The price is affordable. I will keep using it.
Date published: 2024-09-26
Rated 5 out of 5 by from Very GoodVery Good product. I was able to yield good results using this.
Date published: 2024-09-13
Rated 5 out of 5 by from Amazing Pull DownProtein A/G PLUS-Agarose is an amazing bead for the IP procedures, for the given price.
Date published: 2024-06-05
Rated 5 out of 5 by from Good Protein A/G agarose beadsWe used Protein A/G PLUS-Agarose beads for pull down assays, which works great.
Date published: 2023-05-27
Rated 5 out of 5 by from good beadswe used this to do chip，it is good. We will but it again.
Date published: 2023-02-07
Rated 5 out of 5 by from Great agarose beadIt works pretty well and affordable price. I keep using it more than years.
Date published: 2023-01-20
Rated 5 out of 5 by from 好用我们买了两次，挺好用的。大家可以试一试，不过我们用的比较快，这是第三次了,yuijiangoumai
Date published: 2022-12-19
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Protein A/G PLUS-Agarose is rated 4.9 out of 5 by 160.
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