Protein A-Agarose immunoprecipitation reagent to reduce non-specific immunoglobulin binding

Protein A-Agarose: sc-2001

Protein A-Agarose is rated 4.8 out of 5 by 17.
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Datasheets

    • provided as 0.5 ml agarose/2.0 ml; 100 reactions
    • suitable for use at 20 µl per immunoprecipitation reaction
    • pre-treated to reduce non-specific immunoglobulin binding
    • linked to a high-quality and well established Sepharose resin
    • binding capacity is 10 mg of antibody per 1 ml of packed beads, settled not spun
    • strong binding to mouse IgG2a , IgG2b and IgG3 , rat IgG2c , human IgG1 , IgG2 , IgG4 , rabbit, guinea pig, porcine, canine and feline IgG
    • weak to medium binding to mouse and rat IgG1 , bovine, ovine, and equine IgG
    • pre-washed and ready to use
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How did you pre-treat the beads? Did you use the salmon or other animal's DNA to block the beads? I plan use this kind of beads to do chip sequence, it's will affect my results if the beads contain other animals' DNA.

Asked by: jingwei wang
Thank you for your question. Please contact our Technical Service Department and we can provide you with this information.
Answered by: Technical Support
Date published: 2019-03-05

How do you pre-treat the beads to reduce non-specific binding?I plan to use this beads to do chip-seq.I 'm afraid whether you use ssDNA to pre-treat it, which will affect my chip sequence data.

Asked by: jingwei wang
Thank you for your question. Unfortunately, we don't have an optimized protocol available currently for Chip-seq. Please refer to the following ChIP-seq publication which lists different recipes for blocking agarose beads, as well as a protocol. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665311/
Answered by: Technical Support
Date published: 2019-03-04
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Rated 4 out of 5 by from Protein A agarose Works well in an IP reaction using a Dictyostelium whole cell lysate
Date published: 2020-02-24
Rated 5 out of 5 by from A VERY GOOD REAGENT! In our lab, we did a lot of immuno-precipitation work. We used your protein A/Agarose (sc-2001) for long time, and it works very well in our hand.
Date published: 2019-09-10
Rated 5 out of 5 by from Excelent Product This product is excellent. I am glad that I use this product
Date published: 2019-08-13
Rated 5 out of 5 by from Good product I use these beads for IP in H9 cell line, work very well.
Date published: 2019-07-01
Rated 5 out of 5 by from Works as expected Good price for resin that worked well for small batch purification of an IgG2 fc.
Date published: 2018-08-20
Rated 4 out of 5 by from Worked great Shopped around for a new beads and these did not disappoint
Date published: 2017-09-15
Rated 5 out of 5 by from Used for IP Always usd for IP ,always got good result.I believe this product quality.
Date published: 2017-02-24
Rated 5 out of 5 by from good for ip I bought this for the ip assay, and found it works well. good.
Date published: 2016-11-21
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