Propidium Iodide CAS: 25535-16-4
MF: C27H34N4•2I
MW: 668.39

Propidium Iodide (CAS 25535-16-4)

Propidium Iodide | CAS 25535-16-4 is rated 5.0 out of 5 by 2.
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Alternate Names: Propidium Iodide is also known as Propidium Iodine and 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
Application: Propidium Iodide is a membrane-impermeable fluorescent nucleic acid stain that may be used in flow cytometry to evaluate cell viability when used with ot
CAS Number: 25535-16-4
Purity: ≥94%
Molecular Weight: 668.39
Molecular Formula: C27H34N42I
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Propidium iodide is a laboratory reagent for the fluorescent staining of nucleic acids. Propidium iodide is useful for flow cytometry for staining apoptotic cells and nuclei. Because Propidium iodide is membrane impermeable the state of the cell determines the ability of propidium iodide from staining viable and apoptotic cells. Propidium iodide has been used in in combination with Hoechst fluorescent stain (sc-202503) to observe cell viability via fluorescent microscopy. Propidium iodide is excitable at 536 nm and Emits at 617 nm (red).


References

1. Tas, J. and Westerneng, G. 1981. J. Histochem. Cytochem. 29: 929-936. PMID: 6168679
2. Darzynkiewicz, Z., et al. 1992. Cytometry. 13: 795-808. PMID: 1333943
3. Darzynkiewicz, Z., et al. 1998. Adv. Biochem. Eng. Biotechnol. 62: 33-73. PMID: 9755640
4. Darzynkiewicz, Z. and Bedner, E. 2000. Meth. Enzymol. 322: 18-39. PMID: 10914002

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (1 mg/ml), DMSO, and methanol (3 mg/ml).
Storage :
Store at 4° C
Melting Point :
220-225° C (dec.)
IC50 :
Glutamate NMDA receptor: IC50 = 6.8 µM (Rattus norvegicus); Butyrylcholinesterase: IC50 = 12.6 µM (Equus caballus); Acetylcholinesterase: IC50 = 34.6 µM (Bos taurus); Acetylcholinesterase: IC50 = 7.14 µM (human)
Ki Data :
Acetylcholinesterase: Ki= 8.6 µM (Electrophorus electricus); Acetylcholinesterase: Ki= 3.73 µM (Torpedo californica); Acetylcholinesterase: Ki= 2.23 µM (Mus musculus); Acetylcholinesterase: Ki= 7.14 µM (human)
Fluoresence :
λex 536 nm, λem 617 nm
pK Values :
pKa: 1.63 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
SF7949600
PubChem CID :
104981
MDL Number :
MFCD00011921
EC Number :
247-081-0
Beilstein Registry :
3843838
SMILES :
CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-]

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Certificate of Analysis

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Propidium Iodide (CAS 25535-16-4) Product Citations

See how others have used Propidium Iodide (CAS 25535-16-4). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 80 total

PMID: # 30630060  2019. Eur J Pharm Biopharm. 136: 9-17.

PMID: # 31164357  2019. Cancer Immunol Res. 7: 1120-1134.

PMID: # 31426963  2019. Carbohydr Polym. 223: 115061.

PMID: # 29138869  Rattanawong, A. et al. 2018. Oncol. Rep. 39: 227-238.

PMID: # 28565770  Ma, XW. et al. 2017. Exp Ther Med. 13: 1799-1805.

PMID: # 28759889  Harding, SM.|Benci, JL.|Irianto, J.|Discher, DE.|Minn, AJ.|Greenberg, RA.| et al. 2017. Nature. 548: 466-470.

PMID: # 28948468  Hidalgo-Lanussa, O. et al. 2017. Mol. Neurobiol.

PMID: # 27714810  Puga Molina, LC. et al. 2017. J. Cell. Physiol. 232: 1404-1414.

PMID: # 26676515  Woo, SM. et al. 2016. Mol Med Rep. 13: 1091-6.

PMID: # 26073087  Li, T. et al. 2016. Oncogene. 35: 1575-84.

Citations 1 to 10 of 80 total

What is the appearance of the compound?

Asked by: two2igm05
Thank you for your question. Propidium Iodide, sc-3541, is in red powder form.
Answered by: Chemical Support 4
Date published: 2017-03-21
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Rated 5 out of 5 by from Several publications use propidium iodide Several publications use propidium iodide in their studies to counterstain samples via IHC. -SCBT Publication Review
Date published: 2015-05-21
Rated 5 out of 5 by from Ma Ma, et. al. (PubMed ID 16990610) utilized product sc-3506 to stain cells, in order to study cell cycle distribution. -SCBT Publication Review
Date published: 2015-05-21
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