Pronase E (CAS 9036-06-0)
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Alternate Names:
Pronase E also known as Actinase E; Protease from Streptomyces griseus
Application:
Pronase E is an enzyme used to break down proteins by hydrolyzing peptide bonds in vitro and can be used to study protease inhibitors
CAS Number:
9036-06-0
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Pronase E is an enzyme used to break down proteins by hydrolyzing peptide bonds in vitro. It can degrade proteins and is also used to study protease inhibitors and thermal inactivation kinetics. Pronase E enzyme prefers to cleave peptide bonds on the carboxyl side of glutamic or aspartic acid. Pronase E is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
Pronase E (CAS 9036-06-0) References
- Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska pollack skin. | Kim, SK., et al. 2001. J Agric Food Chem. 49: 1984-9. PMID: 11308357
- Chemical moieties and interactions involved in the binding of zearalenone to the surface of Lactobacillus rhamnosus strains GG. | El-Nezami, H., et al. 2004. J Agric Food Chem. 52: 4577-81. PMID: 15237970
- Combined use of Pronase E and a fungal extract (Penicillium aurantiogriseum) to potentiate the sensory characteristics of dry fermented sausages. | Bruna, JM., et al. 2000. Meat Sci. 54: 135-45. PMID: 22060609
- Voltage-dependent inactivation of the plasmodial surface anion channel via a cleavable cytoplasmic component. | Alkhalil, A., et al. 2012. Biochim Biophys Acta. 1818: 367-74. PMID: 22115742
- Incidence of hemagglutination activity among pathogenic and non-pathogenic Bacteroides fragilis strains and role of capsule and pili in HA and adherence. | Pruzzo, C., et al. 1989. FEMS Microbiol Lett. 50: 113-8. PMID: 2567685
- Release, Separation, and Recovery of Monomeric Reducing N-Glycans with Pronase E Combined with 9-Chloromethyl Chloroformate and Glycosylasparaginase. | Lu, Y., et al. 2019. Biochemistry. 58: 1120-1130. PMID: 30661358
- Cyanobacterial cell-wall components as emerging environmental toxicants - detection and holistic monitoring by cellular signaling biosensors. | Gągała-Borowska, I., et al. 2022. Sci Total Environ. 807: 150645. PMID: 34637876
- Dehydroquinate Synthase Directly Binds to Streptomycin and Regulates Susceptibility of Mycobacterium bovis to Streptomycin in a Non-canonical Mode. | Wei, W., et al. 2022. Front Microbiol. 13: 818881. PMID: 35516432
- An Integrated Strategy to Identify and Quantify the Quality Markers of Xinkeshu Tablets Based on Spectrum-Effect Relationship, Network Pharmacology, Plasma Pharmacochemistry, and Pharmacodynamics of Zebrafish. | Wei, Y., et al. 2022. Front Pharmacol. 13: 899038. PMID: 35677447
- Farnesol is utilized for protein isoprenylation and the biosynthesis of cholesterol in mammalian cells. | Crick, DC., et al. 1995. Biochem Biophys Res Commun. 211: 590-9. PMID: 7794274
- Nuclear transplantation by microinjection of inner cell mass and granulosa cell nuclei. | Collas, P. and Barnes, FL. 1994. Mol Reprod Dev. 38: 264-7. PMID: 7917277
- Utilization of geranylgeraniol for protein isoprenylation in C6 glial cells. | Crick, DC., et al. 1994. Biochem Biophys Res Commun. 205: 955-61. PMID: 7999138
- Attachment of Vibrio alginolyticus to chitin mediated by chitin-binding proteins. | Pruzzo, C., et al. 1996. Microbiology (Reading). 142 (Pt 8): 2181-6. PMID: 8760933
- Production of antifungal substance by Lactococcus lactis subsp. lactis CHD-28.3. | Roy, U., et al. 1996. Int J Food Microbiol. 32: 27-34. PMID: 8880325
- Osteoclastic resorption of biphasic calcium phosphate ceramic in vitro. | Yamada, S., et al. 1997. J Biomed Mater Res. 37: 346-52. PMID: 9368139
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pronase E, 100 mg | sc-264144B | 100 mg | $89.00 | |||
Pronase E, 1 g | sc-264144 | 1 g | $214.00 | |||
Pronase E, 5 g | sc-264144A | 5 g | $658.00 |