Prickle1 Antibody (F-5): sc-393034

Prickle1 Antibody (F-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Prickle1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Prickle1 (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Prickle1, also known as RILP or EPM1B, is an 831 amino acid protein characterized by one PET domain and three LIM zinc-binding domains, and is primarily localized in the cytoplasm and nuclear membrane. This localization suggests a role in cellular signaling and gene regulation, particularly in neuronal function. Prickle1 shows higher expression in placenta and lower expression in liver, brain, kidney, lung, and pancreas, indicating involvement in various physiological processes. Prickle1 functions as a nuclear receptor interacting with neuron-restrictive silencer factor (NRSF), a protein binding to neuron-restrictive silencer element (NRSE) in DNA, playing a significant role in neuronal gene expression regulation. Defects in Prickle1 are linked to autosomal recessive progressive myoclonic epilepsy-1B, marked by involuntary muscle jerks. Prickle1 gene is located on human chromosome 12, encompassing over 1,100 genes and constituting approximately 4.5% of the human genome, highlighting importance in genetic regulation and potential implications in neurological disorders.

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Prickle1 Antibody (F-5) References:

1. Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle.  |  Katoh, M. and Katoh, M. 2003. Int J Mol Med. 11: 249-56. PMID: 12525887
2. Prickle and Strabismus form a functional complex to generate a correct axis during planar cell polarity signaling.  |  Jenny, A., et al. 2003. EMBO J. 22: 4409-20. PMID: 12941693
3. REST/NRSF-interacting LIM domain protein, a putative nuclear translocation receptor.  |  Shimojo, M. and Hersh, LB. 2003. Mol Cell Biol. 23: 9025-31. PMID: 14645515
4. Prickle-1 negatively regulates Wnt/beta-catenin pathway by promoting Dishevelled ubiquitination/degradation in liver cancer.  |  Chan, DW., et al. 2006. Gastroenterology. 131: 1218-27. PMID: 17030191
5. Synergy between the RE-1 silencer of transcription and NFkappaB in the repression of the neurotransmitter gene TAC1 in human mesenchymal stem cells.  |  Greco, SJ., et al. 2007. J Biol Chem. 282: 30039-50. PMID: 17709376

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Prickle1 Antibody (F-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Prickle1 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Prickle1 Antibody (F-5) Specifications

Alternate Product Name: Prickle1

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 685-766

Epitope Target: Prickle1

Epitope Species: human

Product Reactivity/Detection: Prickle1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Prickle1 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin