Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PRC1 Double Nickase Plasmid (h): sc-401679-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PRC1 Double Nickase Plasmid (h) and PRC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PRC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRC1 Antibody (C-1): sc-376983
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRC1 Double Nickase Plasmid (h) | sc-401679-NIC | 20 µg | $410.00 | |||
PRC1 Double Nickase Plasmid (h2) | sc-401679-NIC-2 | 20 µg | $410.00 |
PRC1 (protein regulator of cytokinesis 1) is a conserved microtubule-associated protein that crosslinks antiparallel microtubules in the spindle midzone and is essential for central spindle assembly, cleavage furrow positioning, and completion of cytokinesis. Its activity is coordinated with mitotic kinases and motor proteins to ensure faithful chromosome segregation and abscission, linking PRC1 to core cell cycle and mitotic spindle pathways. Dysregulation of PRC1 expression or function can perturb cell division fidelity and genome stability, features commonly studied in the context of proliferative disorders and tumor biology. PRC1 is therefore widely used as a molecular entry point for dissecting mitotic progression, spindle architecture, and cytokinesis checkpoint control in human cells.
PRC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PRC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PRC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PRC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PRC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.