PPPDE1 Double Nickase Plasmid (h): sc-412095-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PPPDE1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PPPDE1 Double Nickase Plasmid (h) and PPPDE1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DESI2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PPPDE1 Double Nickase Plasmid (h) | sc-412095-NIC | 20 µg | $410.00 | |||
PPPDE1 Double Nickase Plasmid (h2) | sc-412095-NIC-2 | 20 µg | $410.00 |
PPPDE1 (also known as DESI2) encodes a PPPDE-domain–containing protein implicated in ubiquitin-like modifier biology, with reported deSUMOylase/deubiquitinase-like activities that can influence protein stability and signaling output. By modulating post-translational modification states, PPPDE1 is positioned to affect processes such as proteostasis, cellular stress responses, and regulated protein turnover. Altered SUMO/ubiquitin pathway dynamics are frequently associated with dysregulated growth control and genome maintenance, making PPPDE1 a useful target for mechanistic studies in disease-relevant cellular models. Investigating PPPDE1 function can help clarify how modifier processing interfaces with transcriptional regulation and other signaling networks.
PPPDE1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DESI2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DESI2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DESI2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DESI2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.