PPARβ Double Nickase Plasmid (h): sc-400523-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PPARβ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PPARβ Double Nickase Plasmid (h) and PPARβ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PPARD. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PPARβ Antibody (F-10): sc-74517
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PPARβ Double Nickase Plasmid (h) | sc-400523-NIC | 20 µg | $410.00 | |||
PPARβ Double Nickase Plasmid (h2) | sc-400523-NIC-2 | 20 µg | $410.00 |
PPARD encodes peroxisome proliferator-activated receptor beta/delta (PPARβ), a ligand-activated nuclear receptor that heterodimerizes with RXR to regulate transcriptional programs controlling lipid utilization, fatty acid oxidation, and energy homeostasis. PPARβ integrates metabolic and inflammatory cues to coordinate mitochondrial function, oxidative stress responses, and cell fate decisions through PPAR signaling and broader nuclear receptor transcriptional networks. In many cell types, PPARD activity influences glucose and lipid handling as well as cytokine-responsive gene expression, linking it to mechanisms underlying metabolic dysregulation and inflammation-associated phenotypes. Altered PPARD/PPARβ signaling has been investigated in contexts including dyslipidemia, insulin resistance, and tumor biology, where transcriptional rewiring can affect proliferation, differentiation, and microenvironmental interactions.
PPARβ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PPARD locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PPARD. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PPARD function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PPARD-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.