Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PPARα Double Nickase Plasmid (m2): sc-422360-NIC-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PPARα Double Nickase Plasmid (m2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PPARα Double Nickase Plasmid (m2) and PPARα Double Nickase Plasmid (m22) encode distinct paired gRNA designs targeting Ppara. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PPARα Antibody (H-2): sc-398394
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PPARα Double Nickase Plasmid (m2) | sc-422360-NIC-2 | 20 µg | $410.00 |
Mouse Ppara encodes peroxisome proliferator-activated receptor alpha (PPARα), a ligand-activated nuclear receptor that heterodimerizes with RXR to regulate transcription via PPAR response elements. PPARα is a central controller of lipid catabolism, coordinating fatty acid uptake and β-oxidation programs in mitochondria and peroxisomes and integrating metabolic and inflammatory signaling in tissues such as liver, heart, and muscle. Altered Ppara activity is linked to dyslipidemia, hepatic steatosis, insulin resistance, and inflammatory disorders, making it a key target for mechanistic studies of metabolic homeostasis. Gene editing of mouse Ppara supports functional interrogation of nuclear receptor signaling, transcriptional network mapping, and in vivo or cell-based models of lipid metabolism and immunometabolic crosstalk.
PPARα Double Nickase Plasmid (m2) consists of a matched pair of plasmids engineered for high-specificity editing of the Ppara locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ppara. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ppara function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ppara-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.