PPA1 Antibody (42K-7): sc-100823

PPA1 Antibody (42K-7) is a mouse monoclonal IgG1 antibody that detects PPA1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). PPA1, also known as inorganic pyrophosphatase or IOPPP, plays a crucial role in phosphate metabolism by catalyzing the conversion of pyrophosphate to inorganic phosphate, a reaction essential for various cellular processes, including energy metabolism and nucleic acid synthesis. PPA1 is ubiquitously expressed and primarily localized in the cytoplasm, where PPA1 is vital for cell growth and function. PPA1 exists as a homodimer, and PPA1′s enzymatic activity is magnesium-dependent, requiring the binding of two magnesium ions to become active. Each subunit of the homodimer can bind up to four magnesium ions, which enhances the enzyme′s activity and facilitates the formation of complexes with substrates and products, thereby influencing the overall efficiency of phosphate metabolism. PPA1′s activity can be inhibited by calcium, highlighting the importance of ion balance in regulating function. With a critical role in cellular metabolism and growth, PPA1 remains a significant target for research in various biological and medical fields.

The preferred PPA1 antibody is PPA1 Antibody (B-8): sc-377081.