PP5 Antibody (G-6): sc-515257

PP5 Antibody (G-6) is a mouse monoclonal IgG1 antibody that detects PP5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PP5, also known as Protein phosphatase T, plays a crucial role in cellular regulation by dephosphorylating serine residues on various substrates, including skeletal muscle phosphorylase and histone H1, which are vital for processes such as mitosis and RNA biogenesis. Anti-PP5 antibody (G-6) targets a protein predominantly located in the nucleus, where PP5 is involved in the regulation of gene expression and cell cycle progression, making PP5 essential for maintaining cellular homeostasis and proper cell division. The trimeric structure of the PP5 holoenzyme, composed of a regulatory subunit, a variable subunit, and a catalytic subunit, allows for precise control over phosphatase activity, highlighting PP5′s importance in cellular signaling pathways. PP5 (G-6) antibody is an invaluable tool for researchers studying the intricate mechanisms of protein phosphorylation and its implications in various biological processes and diseases.

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PP5 Antibody (G-6) References:

1. Immunohistochemical localization of human pineal tissue antigens in normal retina and retinoblastomas.  |  Sawai, J., et al. 2003. Neuropathology. 23: 119-28. PMID: 12777100
2. Inhibition of mammalian target of rapamycin activates apoptosis signal-regulating kinase 1 signaling by suppressing protein phosphatase 5 activity.  |  Huang, S., et al. 2004. J Biol Chem. 279: 36490-6. PMID: 15218033
3. Constitutive over expression of serine/threonine protein phosphatase 5 (PP5) augments estrogen-dependent tumor growth in mice.  |  Golden, T., et al. 2004. Cancer Lett. 215: 95-100. PMID: 15374638
4. Protein phosphatase 5 is required for ATR-mediated checkpoint activation.  |  Zhang, J., et al. 2005. Mol Cell Biol. 25: 9910-9. PMID: 16260606
5. Hsp90-dependent activation of protein kinases is regulated by chaperone-targeted dephosphorylation of Cdc37.  |  Vaughan, CK., et al. 2008. Mol Cell. 31: 886-95. PMID: 18922470
6. Palbociclib enhances radiosensitivity of hepatocellular carcinoma and cholangiocarcinoma via inhibiting ataxia telangiectasia-mutated kinase-mediated DNA damage response.  |  Huang, CY., et al. 2018. Eur J Cancer. 102: 10-22. PMID: 30103095
7. Exploring the role of active site Mn2+ ions in the binding of protein phosphatase 5 with its substrate using molecular dynamics simulations.  |  Wang, L. and Yan, F. 2019. Biochem Biophys Res Commun. 511: 612-618. PMID: 30826056
8. Production of placental protein 5 (PP5) by non-malignant human fibroblasts in culture. Comparison with 'pregnancy-specific' glycoprotein (SP1).  |  Nisbet, AD., et al. 1983. Oncodev Biol Med. 4: 281-8. PMID: 6601264
9. Cloning of a highly conserved human protein serine-threonine phosphatase gene from the glioma candidate region on chromosome 19q13.3.  |  Yong, WH., et al. 1995. Genomics. 29: 533-6. PMID: 8666404
10. Purification and identification of a novel and four known serine proteinase inhibitors secreted by human glioblastoma cells.  |  Koshikawa, N., et al. 1996. J Biochem. 119: 334-9. PMID: 8882727

The preferred PP5 antibody is PP5 Antibody (H-7): sc-271816.