Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PP1γ Double Nickase Plasmid (h): sc-401476-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PP1γ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PP1γ Double Nickase Plasmid (h) and PP1γ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PPP1CC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PP1γ Antibody (E-4): sc-515943
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PP1γ Double Nickase Plasmid (h) | sc-401476-NIC | 20 µg | $410.00 | |||
PP1γ Double Nickase Plasmid (h2) | sc-401476-NIC-2 | 20 µg | $410.00 |
PPP1CC encodes the catalytic subunit protein phosphatase 1 gamma (PP1γ), a major serine/threonine phosphatase that counterbalances kinase signaling by dephosphorylating diverse substrates in the nucleus and cytoplasm. PP1γ activity is directed by regulatory subunits to control cell-cycle progression, mitotic exit, DNA damage responses, glycogen metabolism, and cytoskeletal dynamics. Through these pathways, PPP1CC helps tune phosphorylation-dependent processes such as chromatin organization, transcriptional regulation, and centrosome/spindle function. Dysregulated PP1 signaling and altered PPP1CC expression or localization have been linked to proliferative and stress-response phenotypes relevant to cancer biology, neurobiology, and metabolic regulation.
PP1γ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PPP1CC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PPP1CC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PPP1CC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PPP1CC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.