PP1α Double Nickase Plasmid (h): sc-400318-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PP1α Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PP1α Double Nickase Plasmid (h) and PP1α Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PPP1CA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PP1 Antibody (E-9): sc-7482
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PP1α Double Nickase Plasmid (h) | sc-400318-NIC | 20 µg | $410.00 | |||
PP1α Double Nickase Plasmid (h2) | sc-400318-NIC-2 | 20 µg | $410.00 |
PPP1CA encodes the catalytic subunit alpha isoform of protein phosphatase 1 (PP1α), a major serine/threonine phosphatase that reverses phosphorylation events across diverse cellular programs. PP1α activity is directed by regulatory subunits to control glycogen metabolism, cell-cycle progression, chromatin dynamics, and cytoskeletal organization, counterbalancing kinase-driven signaling. Through dephosphorylation of key substrates, PPP1CA helps coordinate DNA damage responses, mitotic exit, and transcriptional regulation. Dysregulated PP1 signaling and altered phosphatase–regulator interactions have been linked to aberrant proliferation and stress signaling observed in multiple disease-associated cellular states, supporting its study in pathway-focused mechanistic models.
PP1α Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PPP1CA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PPP1CA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PPP1CA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PPP1CA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.