Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PON2 Double Nickase Plasmid (h): sc-403181-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PON2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PON2 Double Nickase Plasmid (h) and PON2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PON2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PON2 Antibody (C-5): sc-374158
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PON2 Double Nickase Plasmid (h) | sc-403181-NIC | 20 µg | $410.00 |
Paraoxonase 2 (PON2) is a ubiquitously expressed, membrane-associated lactonase that limits oxidative stress by reducing lipid peroxidation and modulating cellular redox homeostasis. It localizes primarily to the endoplasmic reticulum and mitochondria, where it influences reactive oxygen species handling, inflammatory signaling, and survival pathways linked to mitochondrial function. PON2 activity has been connected to regulation of lipid metabolism and cellular stress responses that shape susceptibility to metabolic and vascular dysfunction. Altered PON2 expression or function has been reported in contexts of atherosclerosis, neurodegeneration, and tumor biology, supporting its relevance for mechanistic studies of oxidative injury and inflammation.
PON2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PON2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PON2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PON2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PON2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.