Polyoma virus middle T antigen Antibody (PyMT): sc-53481

Polyoma virus middle T antigen Antibody (PyMT) is a rat monoclonal IgG2b antibody that detects Polyoma virus middle T antigen of Polyoma Virus origin by immunoprecipitation (IP) and immunofluorescence (IF). Anti-Polyoma virus middle T antigen antibody (PyMT) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Polyoma virus is a small oncogenic DNA virus belonging to the family Polymaviridae, known for inducing multiple tumors in infected hosts. Middle T antigen, comprising 421 amino acids, is a crucial transforming protein that plays a significant role in cellular transformation and is essential for the transformation process. Middle T antigen is divided into at least three functional domains, some overlapping with large T antigen and small T antigen domains, highlighting complex interactions within the viral life cycle. Middle T antigen activates various signaling pathways that promote cell proliferation and survival, making this protein a key player in oncogenesis. Understanding middle T antigen function is vital for developing targeted therapies and interventions in viral-induced cancers, as this research provides insights into tumorigenesis mechanisms and potential therapeutic targets.

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Polyoma virus middle T antigen Antibody (PyMT) References:

1. Polyoma virus middle T antigen and its role in identifying cancer-related molecules.  |  Dilworth, SM. 2002. Nat Rev Cancer. 2: 951-6. PMID: 12459733
2. Mechanisms of transformation by polyoma virus middle T antigen.  |  Kaplan, DR., et al. 1989. Biochim Biophys Acta. 948: 345-64. PMID: 2465782
3. Expression of biologically active middle T antigen of polyoma virus from recombinant baculoviruses.  |  Forstová, J., et al. 1989. Nucleic Acids Res. 17: 1427-43. PMID: 2646594
4. Expression of polyoma early gene products in E. coli.  |  Schaffhausen, B., et al. 1985. Nucleic Acids Res. 13: 501-19. PMID: 2987799
5. Large T antigens of simian virus 40 and polyomavirus efficiently establish primary fibroblasts.  |  Jat, PS. and Sharp, PA. 1986. J Virol. 59: 746-50. PMID: 3016337
6. Polyomavirus small T antigen enhances replication of viral genomes in 3T6 mouse fibroblasts.  |  Berger, H. and Wintersberger, E. 1986. J Virol. 60: 768-70. PMID: 3022009
7. Binding of polyomavirus large T antigen to the human hsp70 promoter is not required for trans activation.  |  Kingston, RE., et al. 1986. Mol Cell Biol. 6: 3180-90. PMID: 3023966
8. Monoclonal antibodies against polyoma virus tumor antigens.  |  Dilworth, SM. and Griffin, BE. 1982. Proc Natl Acad Sci U S A. 79: 1059-63. PMID: 6175960
9. The complex of polyoma virus middle-T antigen and pp60c-src.  |  Courtneidge, SA. and Smith, AE. 1984. EMBO J. 3: 585-91. PMID: 6325177
10. Protein kinase activities associated with distinct antigenic forms of polyoma virus middle T-antigen.  |  Dilworth, SM. 1982. EMBO J. 1: 1319-28. PMID: 6327264
11. The polyoma virus T antigen interferes with interferon-inducible gene expression.  |  Weihua, X., et al. 1998. Proc Natl Acad Sci U S A. 95: 1085-90. PMID: 9448289
12. Polyomavirus large T antigen binds the transcriptional coactivator protein p300.  |  Nemethova, M. and Wintersberger, E. 1999. J Virol. 73: 1734-9. PMID: 9882390

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Polyoma virus middle T antigen Antibody (PyMT) has 19 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Polyoma virus middle T antigen Antibody (PyMT) Specifications

Alternate Product Name: Polyoma virus middle T antigen

Product Clone Name: PyMT

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: Polyoma virus- middle T antigen

Epitope Species: Polyoma Virus

Product Reactivity/Detection: Polyoma virus- middle T antigen

Species Reactivity/Detection: Polyoma Virus

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IF

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for Polyoma virus middle T antigen Antibody (PyMT): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin