Polyoma virus large T antigen Antibody (PyLT): sc-53479

Polyoma virus large T antigen Antibody (PyLT) is a rat monoclonal IgG2b antibody that detects the Polyoma virus large T antigen of Polyoma Virus origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Polyoma virus large T antigen antibody (PyLT) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Polyoma virus is a small oncogenic DNA virus belonging to the family Polymaviridae, known for inducing multiple tumors in infected hosts. The large T antigen plays a crucial role in the viral life cycle as a nuclear phosphoprotein that regulates viral replication and gene expression while facilitating the isolation of viral T antigens. Polyoma virus large T antigen can induce cellular DNA replication independently of other virus-transforming genes, highlighting significance in the transformation process. The large T antigen interacts with several cellular proteins, including the retinoblastoma protein (Rb) and p53, which are critical regulators of the cell cycle and apoptosis. By binding to these proteins, the large T antigen disrupts normal functions, leading to uncontrolled cell proliferation and contributing to the oncogenic potential of the virus. Polyoma virus large T antigen monoclonal antibody (PyLT) serves as an essential tool for researchers studying viral oncogenesis and the molecular mechanisms underlying tumor development.

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Polyoma virus large T antigen Antibody (PyLT) References:

1. A tumor host range selection procedure identifies p150(sal2) as a target of polyoma virus large T antigen.  |  Li, D., et al. 2001. Proc Natl Acad Sci U S A. 98: 14619-24. PMID: 11734654
2. Nephron segment localization of polyoma virus large T antigen in renal allografts.  |  Meehan, SM., et al. 2006. Hum Pathol. 37: 1400-6. PMID: 16949647
3. Characterization of functional domains in the Merkel cell polyoma virus Large T antigen.  |  Houben, R., et al. 2015. Int J Cancer. 136: E290-300. PMID: 25208506
4. Expression of biologically active middle T antigen of polyoma virus from recombinant baculoviruses.  |  Forstová, J., et al. 1989. Nucleic Acids Res. 17: 1427-43. PMID: 2646594
5. Expression of polyoma early gene products in E. coli.  |  Schaffhausen, B., et al. 1985. Nucleic Acids Res. 13: 501-19. PMID: 2987799
6. Large T antigens of simian virus 40 and polyomavirus efficiently establish primary fibroblasts.  |  Jat, PS. and Sharp, PA. 1986. J Virol. 59: 746-50. PMID: 3016337
7. Polyomavirus small T antigen enhances replication of viral genomes in 3T6 mouse fibroblasts.  |  Berger, H. and Wintersberger, E. 1986. J Virol. 60: 768-70. PMID: 3022009
8. Binding of polyomavirus large T antigen to the human hsp70 promoter is not required for trans activation.  |  Kingston, RE., et al. 1986. Mol Cell Biol. 6: 3180-90. PMID: 3023966
9. Monoclonal antibodies against polyoma virus tumor antigens.  |  Dilworth, SM. and Griffin, BE. 1982. Proc Natl Acad Sci U S A. 79: 1059-63. PMID: 6175960
10. Protein kinase activities associated with distinct antigenic forms of polyoma virus middle T-antigen.  |  Dilworth, SM. 1982. EMBO J. 1: 1319-28. PMID: 6327264
11. The polyoma virus T antigen interferes with interferon-inducible gene expression.  |  Weihua, X., et al. 1998. Proc Natl Acad Sci U S A. 95: 1085-90. PMID: 9448289
12. Polyomavirus large T antigen binds the transcriptional coactivator protein p300.  |  Nemethova, M. and Wintersberger, E. 1999. J Virol. 73: 1734-9. PMID: 9882390

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Polyoma virus large T antigen Antibody (PyLT) has 1 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Polyoma virus large T antigen Antibody (PyLT) Specifications

Alternate Product Name: Polyoma virus large T antigen

Product Clone Name: PyLT

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: Polyoma virus large T antigen

Epitope Species: Polyoma Virus

Product Reactivity/Detection: Polyoma virus, Large T antigen

Species Reactivity/Detection: Polyoma Virus

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Polyoma virus large T antigen Antibody (PyLT): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin