Polyoma virus early Antibody (PyC): sc-53480

Polyoma virus early Antibody (PyC) is a rat monoclonal IgG2b antibody that detects the Polyoma virus early protein of Polyoma Virus origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Polyoma virus early antibody (PyC) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. The Polyoma virus is a small oncogenic DNA virus belonging to the family Polymaviridae, known for inducing multiple tumors in infected hosts. Among early proteins, the large T antigen plays a crucial role in regulating viral replication and gene expression, vital for viral life cycle and host cell transformation. The large T antigen is a nuclear phosphoprotein that facilitates viral T antigen isolation and induces cellular DNA replication independently of other virus-transforming genes, highlighting importance in both viral pathogenesis and potential therapeutic targets. The middle T antigen, another key player, is responsible for cell transformation and essential for viral oncogenic potential, while the small T antigen contributes to both transformation and productive infection. This multifaceted role of early proteins underscores the significance of Polyoma virus early (PyC) monoclonal antibody in research focused on viral oncogenesis and cellular transformation mechanisms.

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Polyoma virus early Antibody (PyC) References:

1. Expression of biologically active middle T antigen of polyoma virus from recombinant baculoviruses.  |  Forstová, J., et al. 1989. Nucleic Acids Res. 17: 1427-43. PMID: 2646594
2. Expression of polyoma early gene products in E. coli.  |  Schaffhausen, B., et al. 1985. Nucleic Acids Res. 13: 501-19. PMID: 2987799
3. Large T antigens of simian virus 40 and polyomavirus efficiently establish primary fibroblasts.  |  Jat, PS. and Sharp, PA. 1986. J Virol. 59: 746-50. PMID: 3016337
4. Polyomavirus small T antigen enhances replication of viral genomes in 3T6 mouse fibroblasts.  |  Berger, H. and Wintersberger, E. 1986. J Virol. 60: 768-70. PMID: 3022009
5. Binding of polyomavirus large T antigen to the human hsp70 promoter is not required for trans activation.  |  Kingston, RE., et al. 1986. Mol Cell Biol. 6: 3180-90. PMID: 3023966
6. Monoclonal antibodies against polyoma virus tumor antigens.  |  Dilworth, SM. and Griffin, BE. 1982. Proc Natl Acad Sci U S A. 79: 1059-63. PMID: 6175960
7. Protein kinase activities associated with distinct antigenic forms of polyoma virus middle T-antigen.  |  Dilworth, SM. 1982. EMBO J. 1: 1319-28. PMID: 6327264
8. The polyoma virus T antigen interferes with interferon-inducible gene expression.  |  Weihua, X., et al. 1998. Proc Natl Acad Sci U S A. 95: 1085-90. PMID: 9448289
9. Polyomavirus large T antigen binds the transcriptional coactivator protein p300.  |  Nemethova, M. and Wintersberger, E. 1999. J Virol. 73: 1734-9. PMID: 9882390

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Polyoma virus early Antibody (PyC) has 0 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Polyoma virus early Antibody (PyC) Specifications

Alternate Product Name: Polyoma virus early

Product Clone Name: PyC

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: Polyoma virus early

Epitope Species: Polyoma Virus

Product Reactivity/Detection: Polyoma virus early antigens large, middle and small

Species Reactivity/Detection: Polyoma Virus

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Polyoma virus early Antibody (PyC): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin