Date published: 2025-9-15

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Polyacrylamide (CAS 9003-05-8)

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Alternate Names:
PAM
CAS Number:
9003-05-8
Molecular Formula:
(C3H5NO)n
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Polyacrylamide is a colorless, crystalline, solid substance, devoid of any odor, soluble in water, ethanol, and methanol. Due to the presence of a double bond, polyacrylamide is highly reactive, tending to create adducts with different molecules. It is predominantly utilized as a linker for proteins and nucleic acids. Additionally, polyacrylamide finds applications in polyacrylamide gel electrophoresis (PAGE), a widely recognized technique for the analysis of proteins and DNA. It aids in the separation of biomolecules, such as proteins, categorizing them by their sizes. Polyacrylamides are typically anchored on surfaces or membranes either through covalent bonding or absorption.


Polyacrylamide (CAS 9003-05-8) References

  1. Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis.  |  Appella, E., et al. 2000. EXS. 88: 1-27. PMID: 10803368
  2. The use of polyacrylamide gel electrophoresis in animal systematics, phylogeny, and ecophysiology.  |  d'Hondt, JL. 2001. Electrophoresis. 22: 404-12. PMID: 11258746
  3. Polyacrylamide gel electrophoresis of RNA.  |  Rio, DC., et al. 2010. Cold Spring Harb Protoc. 2010: pdb.prot5444. PMID: 20516182
  4. One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE).  |  Brunelle, JL. and Green, R. 2014. Methods Enzymol. 541: 151-9. PMID: 24674069
  5. The No-Nonsens SDS-PAGE.  |  Backman, L. and Persson, K. 2018. Methods Mol Biol. 1721: 89-94. PMID: 29423849
  6. Silver Staining Techniques of Polyacrylamide Gels.  |  Berndt, N., et al. 2018. Methods Mol Biol. 1853: 47-52. PMID: 30097929
  7. Isolation of Proteins from Polyacrylamide Gels.  |  Koristka, S., et al. 2019. Methods Mol Biol. 1855: 461-465. PMID: 30426439
  8. Ultrarapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel Electrophoresis.  |  Thomas, R. and Kurien, BT. 2019. Methods Mol Biol. 1855: 491-494. PMID: 30426443
  9. Polyacrylamide Gel Electrophoresis.  |  Green, MR. and Sambrook, J. 2020. Cold Spring Harb Protoc. 2020: PMID: 33262236
  10. Detection of Radioactive DNA in Polyacrylamide Gels.  |  Green, MR. and Sambrook, J. 2020. Cold Spring Harb Protoc. 2020: PMID: 33262237
  11. Nondenaturing polyacrylamide gel electrophoresis of proteins.  |  Walker, JM. 1994. Methods Mol Biol. 32: 17-22. PMID: 7524940
  12. SDS polyacrylamide gel electrophoresis of proteins.  |  Smith, BJ. 1994. Methods Mol Biol. 32: 23-34. PMID: 7524943
  13. Isoelectric focusing of proteins in ultra-thin polyacrylamide gels.  |  Walker, JM. 1994. Methods Mol Biol. 32: 59-65. PMID: 7951749

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Polyacrylamide, 1 g

sc-476273
1 g
$85.00