POLR3GL Lentiviral Activation Particles (h): sc-413599-LAC

POLR3GL encodes a core subunit of RNA polymerase III that contributes to transcription of small noncoding RNAs, including tRNAs, 5S rRNA, and other Pol III-dependent transcripts essential for protein synthesis capacity and cellular homeostasis. Through regulation of Pol III output, POLR3GL influences growth-related programs, cellular stress responses, and innate immune sensing pathways linked to cytosolic RNA species. Altered Pol III function and dysregulated small-RNA biogenesis have been associated with neurodevelopmental phenotypes and broader mechanisms relevant to transcriptional control in proliferative and metabolically active tissues. POLR3GL therefore serves as a useful node for investigating Pol III transcriptional regulation, RNA metabolism, and downstream effects on cell-state transitions.

POLR3GL Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient POLR3GL upregulation across a broader range of human cell types.

POLR3GL Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the POLR3GL transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous POLR3GL expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native POLR3GL genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.