POL H Antibody (B-7): sc-17770

POL H Antibody (B-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects POL H protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHCP), and enzyme-linked immunosorbent assay (ELISA). POL H (B-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. POL H protein, also known as RAD30A, plays a critical role in DNA repair, specifically in the synthesis of DNA during the replication process following damage. This function is vital because POL H helps maintain genomic stability and prevents mutations that could lead to cancer, particularly in individuals with xeroderma pigmentosum (XP), a genetic disorder characterized by extreme sensitivity to ultraviolet light and a predisposition to skin cancers. Unlike other XP proteins involved in nucleotide excision repair, POL H is essential for translesion synthesis, allowing DNA replication to continue past damaged sites. Deficiencies in POL H lead to a significant reduction in the ability to synthesize intact daughter DNA strands after DNA damage, underscoring POL H′s importance in cellular response to genotoxic stress.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

POL H Antibody (B-7) References:

1. Conserved residues of human XPG protein important for nuclease activity and function in nucleotide excision repair.  |  Constantinou, A., et al. 1999. J Biol Chem. 274: 5637-48. PMID: 10026181
2. The relative expression of mutated XPB genes results in xeroderma pigmentosum/Cockayne's syndrome or trichothiodystrophy cellular phenotypes.  |  Riou, L., et al. 1999. Hum Mol Genet. 8: 1125-33. PMID: 10332046
3. The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta.  |  Masutani, C., et al. 1999. Nature. 399: 700-4. PMID: 10385124
4. [File no. 40. DNA polymerase n (Pol H) in hRad30A. Repair gene].  |  Soussi, T. 2000. Bull Cancer. 87: 769-70. PMID: 11190566
5. Mechanisms by which human cells bypass damaged bases during DNA replication after ultraviolet irradiation.  |  Cleaver, JE. 2002. ScientificWorldJournal. 2: 1296-305. PMID: 12805912
6. Tax impairs DNA replication forks and increases DNA breaks in specific oncogenic genome regions.  |  Chaib-Mezrag, H., et al. 2014. Mol Cancer. 13: 205. PMID: 25185513
7. Predominant role of DNA polymerase eta and p53-dependent translesion synthesis in the survival of ultraviolet-irradiated human cells.  |  Lerner, LK., et al. 2017. Nucleic Acids Res. 45: 1270-1280. PMID: 28180309
8. High incidence of ultraviolet-B-or chemical-carcinogen-induced skin tumours in mice lacking the xeroderma pigmentosum group A gene.  |  Nakane, H., et al. 1995. Nature. 377: 165-8. PMID: 7675085
9. Mutations in XPA that prevent association with ERCC1 are defective in nucleotide excision repair.  |  Li, L., et al. 1995. Mol Cell Biol. 15: 1993-8. PMID: 7891694
10. Identification of a damaged-DNA binding domain of the XPA protein.  |  Kuraoka, I., et al. 1996. Mutat Res. 362: 87-95. PMID: 8538652
11. Separation of protein factors that correct the defects in the seven complementation groups of xeroderma pigmentosum cells.  |  Tateishi, S., et al. 1995. J Biochem. 118: 819-24. PMID: 8576098
12. The DNA helicases acting in nucleotide excision repair, XPD, CSB and XPB, are not required for PCNA-dependent repair of abasic sites.  |  Cappelli, E., et al. 1999. Eur J Biochem. 259: 325-30. PMID: 9914510

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-POL H Antibody (B-7) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-POL H Antibody (B-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

POL H Antibody (B-7) Specifications

Alternate Product Name: DNA Polymerase Eta

Product Clone Name: B-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 414-713

Epitope Target: POL H

Epitope Species: human

Product Reactivity/Detection: POL H

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for POL H Antibody (B-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin