POH1 Antibody (SQ-17): sc-100464

POH1 Antibody (SQ-17) is a mouse monoclonal IgG1 kappa light chain antibody that detects POH1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). POH1 (SQ-17) antibody is available as the non-conjugated form. POH1, a human homolog of the yeast protein Pad1, plays a crucial role in the 26S proteasome, which is essential for the degradation of proteins tagged for destruction via the ubiquitin pathway. Specifically, POH1 is a component of the 19S regulatory cap of the 26S proteasome, where POH1 functions as a deubiquitinase, removing ubiquitin chains from substrates and facilitating their entry into the proteasome′s catalytic core. This process is vital for maintaining cellular protein homeostasis and regulating various cellular functions, including cell cycle progression and stress responses. POH1 is predominantly expressed in heart and skeletal muscle tissues, highlighting POH1′s importance in muscle function and health. Notably, transient overexpression of POH1 in mammalian cells has been shown to confer resistance to chemotherapeutic agents such as taxol and doxorubicin, indicating POH1′s potential role in cancer biology and therapeutic resistance. The gene encoding human POH1 is located on chromosome 2q24.2, and interestingly, the Pad1 homolog found in marine sponges (POHL) is upregulated in response to toxic compounds like staurosporine and taxol. In the parasitic organism Schistosoma mansoni, the Pad1 homolog SmPOH appears to stabilize the transcription factor c-Jun, enhancing c-Jun levels for the transactivation of AP-1-responsive genes, further underscoring the diverse biological significance of POH1 across different species.

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POH1 Antibody (SQ-17) References:

1. Potential multidrug resistance gene POHL: an ecologically relevant indicator in marine sponges.  |  Krasko, A., et al. 2001. Environ Toxicol Chem. 20: 198-204. PMID: 11351409
2. A Schistosoma mansoni Pad1 homologue stabilizes c-Jun.  |  Nabhan, JF., et al. 2001. Mol Biochem Parasitol. 116: 209-18. PMID: 11522353
3. A cryptic protease couples deubiquitination and degradation by the proteasome.  |  Yao, T. and Cohen, RE. 2002. Nature. 419: 403-7. PMID: 12353037
4. Resistance to diverse drugs and ultraviolet light conferred by overexpression of a novel human 26 S proteasome subunit.  |  Spataro, V., et al. 1997. J Biol Chem. 272: 30470-5. PMID: 9374539

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-POH1 Antibody (SQ-17) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-POH1 Antibody (SQ-17) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

POH1 Antibody (SQ-17) Specifications

Alternate Product Name: POH1

Product Clone Name: SQ-17

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-96

Epitope Target: POH1

Epitope Species: human

Product Reactivity/Detection: POH1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for POH1 Antibody (SQ-17): N/A