POFUT2 Antibody (G-1): sc-271239
- POFUT2 Antibody (G-1) is a mouse monoclonal IgG1 κ POFUT2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 198-237 within an internal region of POFUT2 of human origin
- POFUT2 Antibody (G-1) is recommended for detection of POFUT2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and bovine
- Anti-POFUT2 Antibody (G-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for POFUT2 Antibody (G-1) for WB and IHC(P) applications. This reagent is now offered in a bundle with POFUT2 Antibody (G-1) (see ordering information below).
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POFUT2 Antibody (G-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects POFUT2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-POFUT2 antibody (G-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. POFUT2, also known as Peptide-O-fucosyltransferase 2, plays a crucial role in the modification of proteins through the addition of fucose to serine or threonine residues within thrombospondin repeats, which is essential for proper protein function and stability. This post-translational modification influences protein interactions and cellular signaling pathways, thereby impacting various biological processes such as cell adhesion, migration, and immune responses. POFUT2 is localized in the endoplasmic reticulum and exists in three isoforms (A, B, and C), each exhibiting distinct expression patterns that may contribute to functional diversity. Additionally, POFUT2 may possess chaperone-like activity, aiding in the quality control and proper folding of glycoproteins, which is vital for maintaining cellular homeostasis and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
POFUT2 Antibody (G-1) References:
- Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. | Nagase, T., et al. 1999. DNA Res. 6: 63-70. PMID: 10231032
- A new superfamily of protein-O-fucosyltransferases, alpha2-fucosyltransferases, and alpha6-fucosyltransferases: phylogeny and identification of conserved peptide motifs. | Martinez-Duncker, I., et al. 2003. Glycobiology. 13: 1C-5C. PMID: 12966037
- Histology-based expression profiling yields novel prognostic markers in human glioblastoma. | Dong, S., et al. 2005. J Neuropathol Exp Neurol. 64: 948-55. PMID: 16254489
- Protein O-fucosyltransferase 2 adds O-fucose to thrombospondin type 1 repeats. | Luo, Y., et al. 2006. J Biol Chem. 281: 9393-9. PMID: 16464857
- Molecular evolution of protein O-fucosyltransferase genes and splice variants. | Loriol, C., et al. 2006. Glycobiology. 16: 736-47. PMID: 16679357
- Molecular cloning and characterization of a novel human beta1,3-glucosyltransferase, which is localized at the endoplasmic reticulum and glucosylates O-linked fucosylglycan on thrombospondin type 1 repeat domain. | Sato, T., et al. 2006. Glycobiology. 16: 1194-206. PMID: 16899492
- O-fucosylation of thrombospondin type 1 repeats in ADAMTS-like-1/punctin-1 regulates secretion: implications for the ADAMTS superfamily. | Wang, LW., et al. 2007. J Biol Chem. 282: 17024-31. PMID: 17395588
- O-fucosylation is required for ADAMTS13 secretion. | Ricketts, LM., et al. 2007. J Biol Chem. 282: 17014-23. PMID: 17395589
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
POFUT2 Antibody (G-1) | sc-271239 | 200 µg/ml | $322.00 | |||
POFUT2 Antibody (G-1): m-IgG1 BP-HRP Bundle | sc-531685 | 200 µg Ab; 20 µg BP | $361.00 | |||
POFUT2 Antibody (G-1) AC | sc-271239 AC | 500 µg/ml, 25% agarose | $424.00 | |||
POFUT2 Antibody (G-1) HRP | sc-271239 HRP | 200 µg/ml | $322.00 | |||
POFUT2 Antibody (G-1) FITC | sc-271239 FITC | 200 µg/ml | $336.00 | |||
POFUT2 Antibody (G-1) PE | sc-271239 PE | 200 µg/ml | $349.00 | |||
POFUT2 Antibody (G-1) Alexa Fluor® 488 | sc-271239 AF488 | 200 µg/ml | $364.00 | |||
POFUT2 Antibody (G-1) Alexa Fluor® 546 | sc-271239 AF546 | 200 µg/ml | $364.00 | |||
POFUT2 Antibody (G-1) Alexa Fluor® 594 | sc-271239 AF594 | 200 µg/ml | $364.00 | |||
POFUT2 Antibody (G-1) Alexa Fluor® 647 | sc-271239 AF647 | 200 µg/ml | $364.00 | |||
POFUT2 Antibody (G-1) Alexa Fluor® 680 | sc-271239 AF680 | 200 µg/ml | $364.00 | |||
POFUT2 Antibody (G-1) Alexa Fluor® 790 | sc-271239 AF790 | 200 µg/ml | $364.00 | |||
POFUT2 (G-1) Neutralizing Peptide | sc-271239 P | 100 µg/0.5 ml | $69.00 |