Podocalyxin-like 1 Antibody (3D3): sc-23904

Podocalyxin-like 1 Antibody (3D3) is a mouse monoclonal IgG1 kappa light chain antibody raised against human recombinant podocalyxin-like protein, specifically detecting Podocalyxin-like protein 1 (PODXL) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Also known as PCLP1, or simply podocalyxin, PODXL is a transmembrane sialomucin characterized by extensive post-translational modifications, including heavy N- and O-linked glycosylation and sialylation of its extracellular domain. These modifications confer a strong negative charge, contributing to PODXL′s anti-adhesive properties and maintaining the podocyte foot processes essential for the glomerular filtration barrier in the kidneys. The intracellular domain of PODXL contains multiple phosphorylation sites, which play a significant role in intracellular signaling pathways that regulate cell adhesion, migration, and polarity. Beyond the kidneys, PODXL is expressed in various epithelial tissues, including the breast, pancreas, and prostate, as well as in endothelial cells and hemangioblasts, precursors to hematopoietic stem cells. PODXL is involved in cancer progression and metastasis, particularly in breast and prostate cancers, underscoring its clinical relevance in oncology. PODXL interacts with the actin cytoskeleton through association with ezrin, which is essential for maintaining cell shape and polarity, and serves as a ligand for L-selectin, facilitating leukocyte rolling on endothelial cells during inflammatory response. Structurally related to CD34, PODXL mediates interactions between leukocytes and endothelial cells during inflammatory processes. Available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, anti-PODXL antibody (3D3) is a versatile tool for researchers investigating PODXL′s role in physiological and pathological conditions, such as cancer progression and metastasis due to its influence on cell adhesion and migration.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Podocalyxin-like 1 Antibody (3D3) References:

1. Identification of podocalyxin-like protein 1 as a novel cell surface marker for hemangioblasts in the murine aorta-gonad-mesonephros region.  |  Hara, T., et al. 1999. Immunity. 11: 567-78. PMID: 10591182
2. Identification of endoglycan, a member of the CD34/podocalyxin family of sialomucins.  |  Sassetti, C., et al. 2000. J Biol Chem. 275: 9001-10. PMID: 10722749
3. Podocalyxin-like protein 1 expression in primary hepatic tumours and tumour-like lesions.  |  Heukamp, LC., et al. 2006. Histopathology. 49: 242-7. PMID: 16918970
4. Podocalyxin-like protein is expressed in glioblastoma multiforme stem-like cells and is associated with poor outcome.  |  Binder, ZA., et al. 2013. PLoS One. 8: e75945. PMID: 24146797
5. Podocalyxin-like and RNA-binding motif protein 3 are prognostic biomarkers in urothelial bladder cancer: a validatory study.  |  Boman, K., et al. 2017. Biomark Res. 5: 10. PMID: 28293425
6. Prognostic role of podocalyxin-like protein expression in various cancers: A systematic review and meta-analysis.  |  Wang, J., et al. 2017. Oncotarget. 8: 52457-52464. PMID: 28881743
7. Identification of LEA, a podocalyxin-like glycoprotein, as a predictor for the progression of colorectal cancer.  |  Yuan, D., et al. 2018. Cancer Med. 7: 5155-5166. PMID: 30277651
8. Podocalyxin-like protein as a predictive biomarker for benefit of neoadjuvant chemotherapy in resectable gastric and esophageal adenocarcinoma.  |  Borg, D., et al. 2018. J Transl Med. 16: 290. PMID: 30355278
9. Molecular cloning, expression, and characterization of podocalyxin-like protein 1 from rabbit as a transmembrane protein of glomerular podocytes and vascular endothelium.  |  Kershaw, DB., et al. 1995. J Biol Chem. 270: 29439-46. PMID: 7493982
10. Glomerular epithelial protein 1 and podocalyxin-like protein 1 in inflammatory glomerular disease (crescentic nephritis) in rabbit and man.  |  Yang, DH., et al. 1996. Lab Invest. 74: 571-84. PMID: 8600307
11. Sialomucin ligands for selectins: a new family of cell adhesion molecules.  |  Lasky, LA. 1994. Princess Takamatsu Symp. 24: 81-90. PMID: 8983065
12. Identification of podocalyxin-like protein as a high endothelial venule ligand for L-selectin: parallels to CD34.  |  Sassetti, C., et al. 1998. J Exp Med. 187: 1965-75. PMID: 9625756

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Podocalyxin-like 1 Antibody (3D3) has 29 citations in a variety of scientific publications.

Primary antibodies like Anti-Podocalyxin-like 1 Antibody (3D3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Podocalyxin-like 1 Antibody (3D3) Specifications

Alternate Product Name: PODXL

Product Clone Name: 3D3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Podocalyxin-like 1

Epitope Species: human

Product Reactivity/Detection: Podocalyxin-like 1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 29 citations in various scientific publications

Available Conjugates/Forms for Podocalyxin-like 1 Antibody (3D3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin