POD-1 Antibody (A-6): sc-377225

POD-1 Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects POD-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). POD-1 (A-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. POD-1, also known as podocyte-expressed 1, capsulin, or epicardin, is a nuclear basic helix-loop-helix (bHLH) transcription factor that plays a crucial role in the specification of mesodermal cell populations, particularly in the development of the heart, cranial skeletal muscle, gut, and urogenital system. POD-1′s selective expression in mesenchymal cells at sites of epithelial-mesenchymal interaction is vital for the formation of various internal organs, including the kidney, lung, intestine, pancreas, and epicardium, which contributes to coronary artery development. POD-1 is implicated in kidney morphogenesis and spleen organogenesis, and may also influence the development and sex determination of the mammalian gonad. POD-1′s ability to regulate these critical developmental processes underscores POD-1′s importance in both normal physiology and potential pathophysiological conditions, making anti-POD-1 antibody (A-6) an essential tool for researchers studying these intricate biological mechanisms.

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POD-1 Antibody (A-6) References:

1. The basic-helix-loop-helix protein pod1 is critically important for kidney and lung organogenesis.  |  Quaggin, SE., et al. 1999. Development. 126: 5771-83. PMID: 10572052
2. Molecular characterization of mesoderm-restricted basic helix-loop-helix protein, POD-1/Capsulin.  |  Miyagishi, M., et al. 2000. Int J Mol Med. 5: 27-31. PMID: 10601570
3. Cell type-dependent transactivation or repression of mesoderm-restricted basic helix-loop-helix protein, POD-1/Capsulin.  |  Miyagishi, M., et al. 2000. Mol Cell Biochem. 205: 141-7. PMID: 10821432
4. A mean spleen gene.  |  Patterson, M. 2000. Nat Rev Genet. 1: 8. PMID: 11262881
5. Pod-1/Capsulin shows a sex- and stage-dependent expression pattern in the mouse gonad development and represses expression of Ad4BP/SF-1.  |  Tamura, M., et al. 2001. Mech Dev. 102: 135-44. PMID: 11287187
6. POD-1 binding to the E-box sequence inhibits SF-1 and StAR expression in human adrenocortical tumor cells.  |  França, MM., et al. 2013. Mol Cell Endocrinol. 371: 140-7. PMID: 23313103
7. POD-1/TCF21 Reduces SHP Expression, Affecting LRH-1 Regulation and Cell Cycle Balance in Adrenocortical and Hepatocarcinoma Tumor Cells.  |  França, MM., et al. 2015. Biomed Res Int. 2015: 841784. PMID: 26421305
8. New evidences on the regulation of SF-1 expression by POD1/TCF21 in adrenocortical tumor cells.  |  França, MM., et al. 2017. Clinics (Sao Paulo). 72: 391-394. PMID: 28658440
9. Pod-1, a mesoderm-specific basic-helix-loop-helix protein expressed in mesenchymal and glomerular epithelial cells in the developing kidney.  |  Quaggin, SE., et al. 1998. Mech Dev. 71: 37-48. PMID: 9507058
10. Capsulin: a novel bHLH transcription factor expressed in epicardial progenitors and mesenchyme of visceral organs.  |  Lu, J., et al. 1998. Mech Dev. 73: 23-32. PMID: 9545521
11. Cloning of capsulin, a basic helix-loop-helix factor expressed in progenitor cells of the pericardium and the coronary arteries.  |  Hidai, H., et al. 1998. Mech Dev. 73: 33-43. PMID: 9545526
12. epicardin: A novel basic helix-loop-helix transcription factor gene expressed in epicardium, branchial arch myoblasts, and mesenchyme of developing lung, gut, kidney, and gonads.  |  Robb, L., et al. 1998. Dev Dyn. 213: 105-13. PMID: 9733105

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-POD-1 Antibody (A-6) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-POD-1 Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

POD-1 Antibody (A-6) Specifications

Alternate Product Name: POD-1

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 6-80

Epitope Target: POD-1

Epitope Species: human

Product Reactivity/Detection: POD-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for POD-1 Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent