PNPP, Disodium Salt CAS: 4264-83-9
MF: C6H4NO6P•2Na•6H2O
MW: 371.14

PNPP, Disodium Salt (CAS 4264-83-9)

PNPP, Disodium Salt | CAS 4264-83-9 is rated 5.0 out of 5 by 1.
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Alternate Names: PNPP, Disodium Salt is also known as p-Nitrophenyl phosphate disodium hexahydrate.
Application: PNPP, Disodium Salt is an alkaline phosphatase substrate used in ELISA applications while absorbing light at 405nm.
CAS Number: 4264-83-9
Purity: ≥99%
Molecular Weight: 371.14
Molecular Formula: C6H4NO6P•2Na•6H2O
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

A colorimetric alkaline phosphatase soluble substrate, PNPP, Disodium Salt is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures. The reaction with PNPP, Disodium Salt produces a soluble end product that is yellow in color. PNPP, Disodium Salt Can be used to perform kinetic and endpoint determinations. PNPP, Disodium Salt is also used as a phosphorylating agent in nucleotide synthesis. PNPP, Disodium Salt is recommended for use with pNPP Substrate Buffer (sc-296099). Also available in solution form: p-Nitrophenyl Phosphate 50X solution (sc-358718).


References

1. Voller, A., et al. 1976. Bull. World Health Organ. 53: 55-65. PMID: 1085667

2. Engvall, E. 1980. Meth. Enzymol. 70: 419-439. PMID: 6999296

3. Voller, A., et al.1986. Manual of Clinical Laboratory Immunology, 3rd Edition. In Rose, N., et al. eds., Washington, D.C. American Society for Microbiology, 106-107.

Usage :
• To prepare PNPP substrate solution, dissolve 10 mg of room-temperature PNPP, Disodium Salt (sc-3720) into 10 ml of pNPP Substrate Buffer (sc-296099). Mix well and protect from light. Diluted product may be used for up to 10 hours following mixing. Discard if solution becomes yellow or turbid.

• Following incubation of antibody samples and the addition of alkaline phosphatase labeled probes, wash plates thoroughly avoiding the use of phosphate buffers. Add 100 µl of 1X PNPP substrate solution to each well and incubate 15-60 minutes. Incubation times will vary depending on the enzyme present.

• For endpoint analysis, the reaction may be stopped with 3N NaOH and read at 405 nm.
Appearance :
Crystalline powder
Physical State :
Solid
Solubility :
Soluble in water(freely soluble). Insoluble in ethanol, and acetone.
Storage :
Store at -20° C
Melting Point :
>300° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
MDL Number :
MFCD00066288
EC Number :
224-246-5
Beilstein Registry :
5324661
SMILES :
C1=CC(=CC=C1[N+](=O)[O-])OP(=O)([O-])[O-].O.O.O.O.O.O.[Na+].[Na+]

Download SDS (MSDS)

Certificate of Analysis

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PNPP, Disodium Salt (CAS 4264-83-9)  Product Citations

See how others have used PNPP, Disodium Salt (CAS 4264-83-9). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 20 total

PMID: # 29638221  Kawecki, F.|Clafshenkel, WP.|Auger, FA.|Bourget, JM.|Fradette, J.|Devillard, R.| et al. 2018. Biofabrication. 10: 035006.

PMID: # 28625849  Cui, DS.|Beaumont, V.|Ginther, PS.|Lipchock, JM.|Loria, JP.| et al. 2017. J. Mol. Biol. 429: 2360-2372.

PMID: # 28004524  Galbraith, T.|Clafshenkel, WP.|Kawecki, F.|Blanckaert, C.|Labbé, B.|Fortin, M.|Auger, FA.|Fradette, J.| et al. 2017. Adv Healthc Mater. 6:

PMID: # 25643598  Wu, Z. et al. 2015. Biosens Bioelectron. 68: 586-92.

PMID: # 24688547  Kumar, S.|Singh, BR.|Bhardwaj, M.|Singh, V.| et al. 2014. Int J Microbiol. 2014: 238575.

PMID: # 24220608  2014. Mol Med Rep. 9: 293-8.

PMID: # 23831060  Rodríguez-Torres, AM. et al. 2013. FEBS Lett. 587: 2617-22.

PMID: # 19122201  Iuliano, R. et al. 2009. J. Biochem. 145: 377-385.

PMID: # 18976718  Baek, JA. et al. 2009. Nitric Oxide. 20: 122-128.

PMID: # 19142636  Gao, P. et al. 2009. Cancer Immunol. Immunother. 58: 1319-28.

Citations 1 to 10 of 20 total

zebrafis scale도 염색할 수 있나요

Asked by: kkim123
Thank you for question. A colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures. Please contact our Technical Service Department by phone, e-mail or live chat for further detail. 문의 감사합니다. p-Nitrophenyl Phosphate (pNPP) 는 알칼리 포스파타아제의 기질로서 ELISA반응에 쓰이는 알칼리 포스파타아제와 함께 반응하여 색을 나타내는 기질이며 단독염색이 가능한 염색물질이 아닙니다. 더 자세한 정보를 원하시면 저희 기술지원팀에 전화, 이메일또는 온라인채팅으로 연락하기 바랍니다.
Answered by: Technical Support 13
Date published: 2017-02-28
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Rated 5 out of 5 by from Gava et Gava et. al. (PubMed ID 19144997) used pNPP to measure the change in protein tyrosine phosphatase activity. The rate of formation of p-Nitrophenol from pNPP was measured by spectroscopy at 410nm. -SCBT Publication Review
Date published: 2015-04-03
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