(+)-Biotin-(PEO)4-amine (CAS 359860-27-8)

Replication Protein A (RPA) is a single-stranded DNA-binding protein complex essential for various DNA metabolism processes such as replication, repair, and recombination. The RPA complex typically consists of three main subunits: RPA70, RPA32, and RPA14, each with distinct but cooperative functions. RPA70 is primarily responsible for DNA binding and recruitment of other proteins to the DNA, while RPA32 has phosphorylation sites that play a role in cell cycle regulation and DNA damage response. The RPA subunits often work in conjunction with other proteins and enzymes, acting as a scaffold that stabilizes single-stranded DNA and recruits necessary machinery for DNA processes. Phosphorylation or modification of RPA subunits is a common form of regulation, affecting the complex′s affinity for DNA and its interaction with other proteins involved in DNA metabolism. Replication Protein A (RPA) inhibitors comprise a class of molecules designed to disrupt the function of RPA subunits. These subunits are critical components of the RPA complex, which is known for its role in stabilizing single-stranded DNA (ssDNA) and facilitating various DNA metabolic processes such as replication, repair, and recombination. By targeting the RPA complex, these inhibitors act by hindering the RPA-DNA interactions, destabilizing the ssDNA-RPA complex, or preventing phosphorylation events critical for RPA function. Some RPA inhibitors are small molecules that can permeate the cell membrane, while others are larger, more complex structures that operate extracellularly.