Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PLSCR1 Double Nickase Plasmid (h): sc-402203-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PLSCR1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PLSCR1 Double Nickase Plasmid (h) and PLSCR1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PLSCR1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLSCR1 Double Nickase Plasmid (h) | sc-402203-NIC | 20 µg | $410.00 | |||
PLSCR1 Double Nickase Plasmid (h2) | sc-402203-NIC-2 | 20 µg | $410.00 |
PLSCR1 (phospholipid scramblase 1) is a calcium-regulated membrane-associated protein implicated in the bidirectional translocation of phospholipids across the plasma membrane, influencing membrane asymmetry and lipid-dependent signaling. It participates in interferon-stimulated gene networks and can modulate cellular responses linked to innate immunity, apoptosis, and vesicular trafficking. PLSCR1 has been connected to pathways affecting cell growth and differentiation and is frequently studied in contexts of dysregulated inflammatory signaling and oncogenic phenotypes. Altered PLSCR1 expression or function has been reported across multiple disease-associated transcriptomic signatures, making it a useful target for mechanistic studies of membrane dynamics and interferon-driven programs.
PLSCR1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PLSCR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PLSCR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PLSCR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PLSCR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.