plasma kallikrein Double Nickase Plasmid (h): sc-402237-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- plasma kallikrein Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- plasma kallikrein Double Nickase Plasmid (h) and plasma kallikrein Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KLKB1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
plasma kallikrein Double Nickase Plasmid (h) | sc-402237-NIC | 20 µg | $410.00 | |||
plasma kallikrein Double Nickase Plasmid (h2) | sc-402237-NIC-2 | 20 µg | $410.00 |
KLKB1 encodes plasma kallikrein, a serine protease central to the contact activation system and the kallikrein–kinin pathway, where it cleaves high-molecular-weight kininogen to release bradykinin and modulate vascular permeability and inflammatory signaling. Plasma kallikrein activity interfaces with intrinsic coagulation and fibrinolytic processes through reciprocal activation with factor XII, linking proteolysis to hemostatic balance. Dysregulated KLKB1 function has been associated with altered bradykinin generation and contact pathway signaling relevant to inflammatory edema phenotypes and thrombosis-related mechanisms. As a circulating protease with defined substrates and regulatory inhibitors, plasma kallikrein is a useful node for dissecting protease cascades and plasma-derived signaling in endothelial and immune contexts.
plasma kallikrein Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KLKB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KLKB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KLKB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KLKB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.