PKR Antibody (FJ-6) recommended for detection of PKR of human origin by WB, IP, IF, IHC(P) and ELISA

PKR Antibody (FJ-6): sc-100378

PKR Antibody (FJ-6) is rated 4.9 out of 5 by 8.
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Datasheets

    • PKR Antibody (FJ-6) is a mouse monoclonal IgG2a (kappa light chain) provided at 100 µg/ml
    • raised against recombinant PKR of human origin
    • recommended for detection of PKR of human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See PKR (B-10): sc-6282 for PKR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

anti-PKR(FJ-6): sc-100378はmouseで検出できるでしょうか?他の動物種との交差性を教えてください。

Asked by: ウィルス学
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at [email protected], or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メール[email protected]またはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-21

Can sc-100378: PKR (FJ-6) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, sc-100378: PKR (FJ-6) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Great antibody for western analysis This antibody produced a strong signal with little background for the western analysis when used at 1:500 dilution.
Date published: 2019-11-25
Rated 5 out of 5 by from Great monoclonal antibody Robust staining by IHCP and IF. Strong and specific band observed with PKR Antibody (FJ-6)
Date published: 2017-02-08
Rated 5 out of 5 by from Publishable WB data Publishable WB data, successful detection of PKR in human lung epithelial cells (A549). -SCBT Publication Review
Date published: 2015-04-10
Rated 4 out of 5 by from Produced robust immunoperoxidase nuclear Produced robust immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human cerebral cortex tissue. -SCBT QC
Date published: 2015-03-26
Rated 5 out of 5 by from Publishable Western blot results using human Publishable Western blot results using human A549 and PBMCs. -SCBT Publication Review
Date published: 2015-03-11
Rated 5 out of 5 by from WB Published with this antibody using human Huh7 WB Published with this antibody using human Huh7.5.1 cells for immunoblot analysis -SCBT Publication Review
Date published: 2015-02-12
Rated 5 out of 5 by from Precise band obtained in WB using A Precise band obtained in WB using A-431 whole cell lysate. -SCBT QC
Date published: 2015-02-08
Rated 5 out of 5 by from Publishable immunostaining in paraffin embedded Publishable immunostaining in paraffin embedded human mesothelioma tumors. -SCBT Publication Review
Date published: 2015-01-11
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