PKR Antibody (13): sc-136038

PKR Antibody (13) is a mouse monoclonal IgG1 antibody that detects PKR in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). PKR, or interferon-inducible RNA-dependent protein serine/threonine kinase, plays a crucial role in the cellular response to viral infections by phosphorylating the α subunit of the protein synthesis initiation factor eIF-2. This phosphorylation event on serine-51 leads to the inhibition of translation, effectively halting viral replication and promoting an antiviral state within the cell. PKR is primarily located in the cytoplasm, where PKR can rapidly respond to the presence of double-stranded RNA, a common indicator of viral infection. The structural organization of PKR includes distinct functional domains, with the RNA-binding domains situated in the amino terminal region and the kinase catalytic domains located in the carboxy terminal half. This arrangement is vital for regulatory mechanisms, which include transcriptional activation in response to interferon, an autoregulatory feedback loop controlling PKR expression, and post-translational modifications such as RNA-mediated autophosphorylation. Anti-PKR antibody (13) is an essential tool for researchers studying the intricate pathways of immune response and viral pathogenesis.

The preferred PKR antibody is PKR Antibody (B-10): sc-6282.