PKLR Antibody (D-10): sc-166228

PKLR Antibody (D-10) is a mouse monoclonal IgG2b antibody that detects pyruvate kinase, liver and RBC (PKLR) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PKLR plays a crucial role in cellular metabolism by catalyzing the final step of glycolysis, converting phosphoenolpyruvate to pyruvate while generating adenosine triphosphate (ATP), which is essential for energy production in cells. Anti-PKLR antibody (D-10) is vital for research, as deficiencies or mutations in this enzyme can lead to serious conditions such as chronic nonspherocytic hemolytic anemia and maturity-onset diabetes of the young, highlighting its importance in both metabolic pathways and disease states. The PKLR gene encodes two isoforms, the L-type and R-type, through alternative splicing, with the R-type predominantly expressed in red blood cells and the L-type in the liver, further emphasizing the significance of this protein in different tissues and its regulatory mechanisms in response to glucose levels. PKLR (D-10) antibody is an invaluable tool for researchers studying glycolytic pathways, metabolic disorders, and the physiological roles of pyruvate kinase in various biological contexts.

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PKLR Antibody (D-10) References:

1. PKLR- GBA region shows almost complete linkage disequilibrium over 70 kb in a set of worldwide populations.  |  Mateu, E., et al. 2002. Hum Genet. 110: 532-44. PMID: 12107439
2. Disruption of a novel regulatory element in the erythroid-specific promoter of the human PKLR gene causes severe pyruvate kinase deficiency.  |  van Wijk, R., et al. 2003. Blood. 101: 1596-602. PMID: 12393511
3. Lack of association between PKLR rs3020781 and NOS1AP rs7538490 and type 2 diabetes, overweight, obesity and related metabolic phenotypes in a Danish large-scale study: case-control studies and analyses of quantitative traits.  |  Andreasen, CH., et al. 2008. BMC Med Genet. 9: 118. PMID: 19111066
4. Novel type of red blood cell pyruvate kinase hyperactivity predicts a remote regulatory locus involved in PKLR gene expression.  |  van Oirschot, BA., et al. 2014. Am J Hematol. 89: 380-4. PMID: 24375447
5. Two Novel Missense Mutations and a 5bp Deletion in the Erythroid-Specific Promoter of the PKLR Gene in Two Unrelated Patients With Pyruvate Kinase Deficient Transfusion-Dependent Chronic Nonspherocytic Hemolytic Anemia.  |  Kager, L., et al. 2016. Pediatr Blood Cancer. 63: 914-6. PMID: 26728349
6. Molecular basis of pyruvate kinase deficiency among Tunisians: description of new mutations affecting coding and noncoding regions in the PKLR gene.  |  Jaouani, M., et al. 2017. Int J Lab Hematol. 39: 223-231. PMID: 28133914
7. Gene editing of PKLR gene in human hematopoietic progenitors through 5' and 3' UTR modified TALEN mRNA.  |  Quintana-Bustamante, O., et al. 2019. PLoS One. 14: e0223775. PMID: 31618280
8. Novel Compound Heterozygous PKLR Mutation Induced Pyruvate Kinase Deficiency With Persistent Pulmonary Hypertension in a Neonate: A Case Report.  |  Lin, S., et al. 2022. Front Cardiovasc Med. 9: 872172. PMID: 35557523
9. Tight linkage of pyruvate kinase (PKLR) and glucocerebrosidase (GBA) genes.  |  Glenn, D., et al. 1994. Hum Genet. 93: 635-8. PMID: 8005587
10. Linkage disequilibrium of common Gaucher disease mutations with a polymorphic site in the pyruvate kinase (PKLR) gene.  |  Rockah, R., et al. 1998. Am J Med Genet. 78: 233-6. PMID: 9677056

The preferred PKLR antibody is PKLR Antibody (E-2): sc-133222.