Date published: 2025-9-5

021-6093-6350

SCBT Portrait Logo
Seach Input

PKI (5-24), PKA Inhibitor 抑制剂 (CAS 99534-03-9)

5.0(1)
寫評論提問

查看产品引用文献(15)

备用名:
L-threonyl-L-threonyl-L-tyrosyl-L-alanyl-L-a-aspartyl-L-phenylalanyl-L-isoleucyl-L-alanyl-L-serylgl ycyl-L-arginyl-L-threonylglycyl-larginyl-L-aspartic acid
应用:
PKI (5-24), PKA Inhibitor 是一种强效、竞争性合成肽 PKA 抑制剂
CAS号码:
99534-03-9
纯度:
≥95%
分子量:
2222.37
分子式:
C94H148N32O31
仅供科研使用。不可用于诊断或治疗。
* 参考分析证明 大量特定数据 (包括水 含量).

快捷链接

PKI (5-24)是PKA(环腺苷酸依赖性蛋白激酶)的强效、竞争性、合成肽抑制剂,它来源于自然发生的热稳定抑制剂蛋白PKI的活性域。这种伪底物抑制剂肽通过精氨酸簇基本亚位点与催化位点结合,模拟蛋白质底物,提供了高特异性。PKA催化亚基酪氨酸235和苯丙氨酸239与PKI (5-24), PKA Inhibitor的苯丙氨酸10形成三明治样结构;这是一个突出的酶-底物相互作用位点。对于PKA,PKI (5-24), PKA Inhibitor的Ki值为2.3 nM,并且它是cGMP依赖性蛋白激酶(PKG)的极弱抑制剂。


PKI (5-24), PKA Inhibitor 抑制剂 (CAS 99534-03-9) 参考文献

  1. cGMP 在透化小鼠主动脉中主要通过 cAMP 激酶发出信号。  |  Wörner, R., et al. 2007. Am J Physiol Heart Circ Physiol. 292: H237-44. PMID: 16920816
  2. 与单磷酸环腺苷依赖性蛋白激酶催化亚基结合的肽抑制剂的结构。  |  Knighton, DR., et al. 1991. Science. 253: 414-20. PMID: 1862343
  3. 一种有效的 cAMP 依赖性蛋白激酶合成肽抑制剂。  |  Cheng, HC., et al. 1986. J Biol Chem. 261: 989-92. PMID: 3511044
  4. cAMP 在去膜公羊精子再活化中的作用  |  San Agustin, JT. and Witman, GB. 1994. Cell Motil Cytoskeleton. 27: 206-18. PMID: 8020107
  5. 从猪心 cAMP 依赖性蛋白激酶催化亚基与 Mn2+ 腺苷酰亚胺二磷酸及抑制剂肽 PKI(5-24) 复合物的 2.0 A 结构推导出的磷酸转移酶和底物结合机制。  |  Bossemeyer, D., et al. 1993. EMBO J. 12: 849-59. PMID: 8384554
  6. 一种 cGMP 激酶突变体,对蛋白激酶抑制剂肽 PKI(5-24) 的敏感性增加。  |  Ruth, P., et al. 1996. Biol Chem. 377: 513-20. PMID: 8922286
  7. 海洋甲藻中 cAMP 依赖性蛋白激酶及其与蛋白磷酸酶-1 可能的直接相互作用的鉴定和特征描述。  |  Dawson, JF., et al. 1996. Biochem Cell Biol. 74: 559-67. PMID: 8960362
  8. 锚定 cAMP 依赖性蛋白激酶对克隆骨骼肌 L 型 Ca2+ 通道的调控  |  Johnson, BD., et al. 1997. J Neurosci. 17: 1243-55. PMID: 9006969
  9. 多聚组氨酸标记的重组 cAMP 依赖性蛋白激酶催化亚基与多肽抑制剂 PKI(5-24) 和腺苷复合物的晶体结构。  |  Narayana, N., et al. 1997. Biochemistry. 36: 4438-48. PMID: 9109651
  10. cAMP 依赖性蛋白磷酸化对原生 Kv1.3 通道的调控。  |  Chung, I. and Schlichter, LC. 1997. Am J Physiol. 273: C622-33. PMID: 9277360
  11. 通过降低酰胺质子的溶剂可及性来识别蛋白质-蛋白质界面。  |  Mandell, JG., et al. 1998. Proc Natl Acad Sci U S A. 95: 14705-10. PMID: 9843953

订购信息

产品名称产品编号规格价格数量收藏夹

PKI (5-24), PKA Inhibitor, 500 µg

sc-201159
500 µg
$117.00

What is the difference between these 2 reagents: 1-PKI (5-24), PKA Inhibitor (CAS 99534-03-9) 2-PKA Inhibitor IV (CAS 99534-03-9)

Asked by: Moustafahamouly
Thank you for your question. There is no difference between these two chemicals. We will look into discontinuing one from our catalog.
Answered by: Technical Support
Date published: 2019-03-10

Is PKA Inhibitor (5-24) membrane permeable?

Asked by: Ivan Goussakov
Thank you for your question. This biochemical is not cell permeable. Please contact Technical Service if you have any further questions or need additional information.
Answered by: Tech Service
Date published: 2018-11-30

Is it cell permeable?

Asked by: ascientist
Thank you for your question. We do not have any data to support this biochemical, PKI (5-24), PKA Inhibitor (CAS 99534-03-9): sc-201159, being cell permeable. We do have a reference in the primary literature which describes how this biochemical can be altered (through conjugation with a peptide) to become cell permeable. If you have more questions about this, please refer to this publication on NCBI's PubMed: PMID: 16761111.
Answered by: Technical Support
Date published: 2024-07-18
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_201159, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 103ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Westphal et alWestphal et al. (PubMed ID 10390370) used the PKA inhibitor, PKI (5-24) (PKI), to prevent cAMP from enhancing NMDA currents. -SCBT Publication Review
Date published: 2015-06-07
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_zh_CN, sid_201159, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT
PKI (5-24), PKA Inhibitor 抑制剂 is rated 5.0 out of 5 by 1.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_zh_CN, sid_201159, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 117ms
  • REVIEWS, PRODUCT