Molecular structure of PKI (5-24), PKA Inhibitor, CAS Number: 99534-03-9
PKI (5-24), PKA Inhibitor Inhibitor (CAS 99534-03-9)
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Alternate Names:
L-threonyl-L-threonyl-L-tyrosyl-L-alanyl-L-a-aspartyl-L-phenylalanyl-L-isoleucyl-L-alanyl-L-serylgl ycyl-L-arginyl-L-threonylglycyl-larginyl-L-aspartic acid
Application:
PKI (5-24), PKA Inhibitor is a potent, competitive, synthetic peptide PKA inhibitor
CAS Number:
99534-03-9
Purity:
≥95%
Molecular Weight:
2222.37
Molecular Formula:
C94H148N32O31
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
PKI (5-24), PKA Inhibitor is a potent, competitive, synthetic peptide inhibitor of PKA (cAMP-dependent protein kinase) derived from the active domain of the naturally-occurring heat-stable inhibitor protein PKI. This pseudosubstrate inhibitor peptide mimics the protein substrate by binding to the catalytic site via the arginine-cluster basic subsite, which provides high specificity. PKA catalytic subunit residues Tyr235 and Phe239 form a sandwich-like structure with residue Phe10 of PKI (5-24), PKA Inhibitor this is a prominent enzyme-substrate interaction site. PKI (5-24), PKA Inhibitor exhibits a Ki of 2.3 nM with respect to PKA and is an extremely weak inhibitor of cGMP-dependent protein kinase (PKG).
PKI (5-24), PKA Inhibitor Inhibitor (CAS 99534-03-9) References
- cGMP signals mainly through cAMP kinase in permeabilized murine aorta. | Wörner, R., et al. 2007. Am J Physiol Heart Circ Physiol. 292: H237-44. PMID: 16920816
- Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. | Knighton, DR., et al. 1991. Science. 253: 414-20. PMID: 1862343
- A potent synthetic peptide inhibitor of the cAMP-dependent protein kinase. | Cheng, HC., et al. 1986. J Biol Chem. 261: 989-92. PMID: 3511044
- Role of cAMP in the reactivation of demembranated ram spermatozoa. | San Agustin, JT. and Witman, GB. 1994. Cell Motil Cytoskeleton. 27: 206-18. PMID: 8020107
- Phosphotransferase and substrate binding mechanism of the cAMP-dependent protein kinase catalytic subunit from porcine heart as deduced from the 2.0 A structure of the complex with Mn2+ adenylyl imidodiphosphate and inhibitor peptide PKI(5-24). | Bossemeyer, D., et al. 1993. EMBO J. 12: 849-59. PMID: 8384554
- A cGMP kinase mutant with increased sensitivity to the protein kinase inhibitor peptide PKI(5-24). | Ruth, P., et al. 1996. Biol Chem. 377: 513-20. PMID: 8922286
- Identification and characterization of cAMP-dependent protein kinase and its possible direct interactions with protein phosphatase-1 in marine dinoflagellates. | Dawson, JF., et al. 1996. Biochem Cell Biol. 74: 559-67. PMID: 8960362
- Modulation of the cloned skeletal muscle L-type Ca2+ channel by anchored cAMP-dependent protein kinase. | Johnson, BD., et al. 1997. J Neurosci. 17: 1243-55. PMID: 9006969
- Crystal structure of a polyhistidine-tagged recombinant catalytic subunit of cAMP-dependent protein kinase complexed with the peptide inhibitor PKI(5-24) and adenosine. | Narayana, N., et al. 1997. Biochemistry. 36: 4438-48. PMID: 9109651
- Regulation of native Kv1.3 channels by cAMP-dependent protein phosphorylation. | Chung, I. and Schlichter, LC. 1997. Am J Physiol. 273: C622-33. PMID: 9277360
- Identification of protein-protein interfaces by decreased amide proton solvent accessibility. | Mandell, JG., et al. 1998. Proc Natl Acad Sci U S A. 95: 14705-10. PMID: 9843953
Inhibitor of:
PKA, and Ser/Thr Protein Kinase.Substrate of:
Enzyme.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKI (5-24), PKA Inhibitor, 500 µg | sc-201159 | 500 µg | $255.00 |