PKC theta substrate-Biotinylated: sc-3107

The PKC θ substrate-Biotinylated is a chemical compound used extensively in scientific research to study the phosphorylation activity of protein kinase C theta (PKC θ), a member of the protein kinase C (PKC) family. This substrate is typically a peptide sequence containing specific amino acid residues that serve as targets for PKC θ-mediated phosphorylation. The addition of a biotin moiety allows for the detection and quantification of phosphorylated peptides using streptavidin-based detection methods, such as enzyme-linked immunosorbent assays (ELISAs) or Western blotting. Researchers utilize the PKC θ substrate-Biotinylated to investigate the substrate specificity, kinetics, and regulation of PKC θ activity in various cellular processes, including immune cell signaling, T cell activation, and inflammation. Furthermore, this chemical tool has been employed in studies aiming to explain the role of PKC θ in the pathogenesis of autoimmune diseases, cancer, and inflammatory disorders. By using the PKC θ substrate-Biotinylated, scientists can gain insights into the molecular mechanisms underlying PKC θ-mediated signaling pathways.