PKC mu Antibody (G-9): sc-518065
- PKC mu Antibody (G-9) is a mouse monoclonal IgM PKC mu antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 375-399 within an internal region of PKC μ of human origin
- PKC mu Antibody (G-9) is recommended for detection of PKC μ of human origin by WB, IP, IF and ELISA
- Anti-PKC mu Antibody (G-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PKC mu Antibody (G-9). This work is in progress.
PKC μ Antibody (G-9) is a mouse monoclonal IgM antibody that detects PKC μ protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PKC μ (G-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PKC μ protein, also known as protein kinase D1 (PRKD1), plays a crucial role in cellular signaling pathways, particularly in regulating cell growth, differentiation, and survival. PKC μ protein is primarily located in the cytoplasm and can translocate to the membrane in response to specific stimuli, which is vital for mediating cellular responses to growth factors and hormones. Proper localization of PKC μ is essential for activation and subsequent signaling cascade, as PKC μ interacts with various membrane-associated proteins and lipids, thereby influencing processes such as gene expression and apoptosis. Understanding PKC μ localization and interactions is important for elucidating PKC μ′s role in cancer progression and other diseases, making PKC μ (G-9) antibody a valuable tool for researchers studying these critical pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PKC mu Antibody (G-9) References:
- A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle. | Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
- Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC. | Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
- Expression and characterization of protein kinase C-delta. | Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
- Loss of PKC mu function induces cytoskeletal defects in mouse oocyte meiosis. | Zhang, Y., et al. 2019. J Cell Physiol. 234: 18513-18523. PMID: 30912144
- The μ-opioid receptor induces miR-21 expression and is ERK/PKCμ-dependent. | Chang, JK., et al. 2021. J Neuroimmunol. 356: 577585. PMID: 33940234
- IL-33 via PKCμ/PRKD1 Mediated α-Catenin Phosphorylation Regulates Endothelial Cell-Barrier Integrity and Ischemia-Induced Vascular Leakage. | Sharma, D., et al. 2023. Cells. 12: PMID: 36899839
- Gαq-PKD/PKCμ signal regulating the nuclear export of HDAC5 to induce the IκB expression and limit the NF-κB-mediated inflammatory response essential for early pregnancy. | Jiang, Y., et al. 2023. Elife. 12: PMID: 37498654
- Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids. | Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
- Turnover of inositol phospholipids and signal transduction. | Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
- The role of protein kinase C in cell surface signal transduction and tumour promotion. | Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
- Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters. | Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
- Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. | Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKC mu Antibody (G-9) | sc-518065 | 200 µg/ml | $316.00 | |||
PKC mu Antibody (G-9) AC | sc-518065 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PKC mu Antibody (G-9) HRP | sc-518065 HRP | 200 µg/ml | $316.00 | |||
PKC mu Antibody (G-9) FITC | sc-518065 FITC | 200 µg/ml | $330.00 | |||
PKC mu Antibody (G-9) PE | sc-518065 PE | 200 µg/ml | $343.00 | |||
PKC mu Antibody (G-9) Alexa Fluor® 488 | sc-518065 AF488 | 200 µg/ml | $357.00 | |||
PKC mu Antibody (G-9) Alexa Fluor® 546 | sc-518065 AF546 | 200 µg/ml | $357.00 | |||
PKC mu Antibody (G-9) Alexa Fluor® 594 | sc-518065 AF594 | 200 µg/ml | $357.00 | |||
PKC mu Antibody (G-9) Alexa Fluor® 647 | sc-518065 AF647 | 200 µg/ml | $357.00 | |||
PKC mu Antibody (G-9) Alexa Fluor® 680 | sc-518065 AF680 | 200 µg/ml | $357.00 | |||
PKC mu Antibody (G-9) Alexa Fluor® 790 | sc-518065 AF790 | 200 µg/ml | $357.00 |