PKC lambda/iota Antibody (E-7): sc-376344
- PKC lambda/iota Antibody (E-7) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 191-229 within an internal region of PKC λ/ι of human origin
- Anti-PKC lambda/iota Antibody (E-7) is recommended for detection of PKC λ/ι of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine and porcine
- Anti-PKC lambda/iota Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PKC lambda/iota Antibody (E-7). This work is in progress.
PKC λ/ι Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PKC λ/ι in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PKC λ/ι (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PKC λ/ι, a member of the protein kinase C (PKC) family, plays a crucial role in cellular signaling pathways that regulate essential processes such as cell growth, differentiation, and apoptosis. A C1 domain that binds diacylglycerol and phorbol esters allows PKC λ/ι to transduce signals in response to growth factors and other stimuli, making PKC λ/ι vital for maintaining cellular homeostasis and responding to external signals. Distinct expression patterns of PKC isoforms across different tissues highlight PKC λ/ι′s importance in specific cellular contexts, as PKC λ/ι activity can influence various downstream effects, including gene expression and metabolic regulation. Understanding PKC λ/ι function and regulation is essential for elucidating PKC λ/ι′s role in health and disease, particularly in cancer and other proliferative disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PKC lambda/iota Antibody (E-7) References:
- Human glioma PKC-iota and PKC-betaII phosphorylate cyclin-dependent kinase activating kinase during the cell cycle. | Acevedo-Duncan, M., et al. 2002. Cell Prolif. 35: 23-36. PMID: 11856176
- Cyclin-dependent kinase activating kinase/Cdk7 co-localizes with PKC-iota in human glioma cells. | Bicaku, E., et al. 2005. Tissue Cell. 37: 53-8. PMID: 15695176
- Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes. | Farese, RV., et al. 2007. J Clin Invest. 117: 2289-301. PMID: 17641777
- Involvement of PKC-iota in glioma proliferation. | Patel, R., et al. 2008. Cell Prolif. 41: 122-35. PMID: 18211289
- Regulation of glioblastoma cell invasion by PKC iota and RhoB. | Baldwin, RM., et al. 2008. Oncogene. 27: 3587-95. PMID: 18212741
- A novel PKC-ι inhibitor abrogates cell proliferation and induces apoptosis in neuroblastoma. | Pillai, P., et al. 2011. Int J Biochem Cell Biol. 43: 784-94. PMID: 21315177
- PKC-ι promotes glioblastoma cell survival by phosphorylating and inhibiting BAD through a phosphatidylinositol 3-kinase pathway. | Desai, S., et al. 2011. Biochim Biophys Acta. 1813: 1190-7. PMID: 21419810
- Regulation of Cdk7 activity through a phosphatidylinositol (3)-kinase/PKC-ι-mediated signaling cascade in glioblastoma. | Desai, SR., et al. 2012. Carcinogenesis. 33: 10-9. PMID: 22021906
- Atypical PKC-iota Controls Stem Cell Expansion via Regulation of the Notch Pathway. | Mah, IK., et al. 2015. Stem Cell Reports. 5: 866-880. PMID: 26527382
- Oncogenic PKC-ι activates Vimentin during epithelial-mesenchymal transition in melanoma; a study based on PKC-ι and PKC-ζ specific inhibitors. | Ratnayake, WS., et al. 2018. Cell Adh Migr. 12: 447-463. PMID: 29781749
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKC lambda/iota Antibody (E-7) | sc-376344 | 200 µg/ml | $316.00 | |||
PKC lambda/iota Antibody (E-7) AC | sc-376344 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PKC lambda/iota Antibody (E-7) HRP | sc-376344 HRP | 200 µg/ml | $316.00 | |||
PKC lambda/iota Antibody (E-7) FITC | sc-376344 FITC | 200 µg/ml | $330.00 | |||
PKC lambda/iota Antibody (E-7) PE | sc-376344 PE | 200 µg/ml | $343.00 | |||
PKC lambda/iota Antibody (E-7) Alexa Fluor® 488 | sc-376344 AF488 | 200 µg/ml | $357.00 | |||
PKC lambda/iota Antibody (E-7) Alexa Fluor® 546 | sc-376344 AF546 | 200 µg/ml | $357.00 | |||
PKC lambda/iota Antibody (E-7) Alexa Fluor® 594 | sc-376344 AF594 | 200 µg/ml | $357.00 | |||
PKC lambda/iota Antibody (E-7) Alexa Fluor® 647 | sc-376344 AF647 | 200 µg/ml | $357.00 | |||
PKC lambda/iota Antibody (E-7) Alexa Fluor® 680 | sc-376344 AF680 | 200 µg/ml | $357.00 | |||
PKC lambda/iota Antibody (E-7) Alexa Fluor® 790 | sc-376344 AF790 | 200 µg/ml | $357.00 | |||
PKC lambda/iota (E-7) Neutralizing Peptide | sc-376344 P | 100 µg/0.5 ml | $68.00 |